Antonie van Leeuwenhoek

, Volume 40, Issue 1, pp 71–78 | Cite as

Variation in the lipid and fatty acid composition in purified membrane fractions from Sarcina aurantiaca in relation to growth phase

  • D. Thirkell
  • Elizabeth M. M. Gray


S. aurantiaca membrane lipid contains both branched and straight-chain fatty acids from C9 to C22 with the saturated branched C15 predominating in almost all of the lipid fractions studied. Unsaturated fatty acids are only present in low concentrations. Significant amounts of straight-chain, even-numbered acids, more common in gram-negative and gram-variable bacteria, are also present. All lipid fractions show a marked change in their fatty acid profiles from the exponential to stationary phase of growth. At least 88% of the total lipid is “free” lipid, and of this material, at least 62% is neutral lipid. The amount of the latter decreases, with a corresponding increase in phospholipid as cells go into stationary phase. During this time, there is a slight fall in the amount of glycolipid which contains predominately mannose, but also glucose and galactose.


Lipid Acid Composition Stationary Phase Fatty Acid Composition Galactose 
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  1. Burchfield, H. P. and Storrs, E. E. 1962. Biochemical applications of gas chromatography, p. 549–554.—Academic Press, London.Google Scholar
  2. Croom, J. A. and McNeil, J. J. 1961. The long chain fatty acids of certain biotin-requiring bacteria.—Bacteriol. Proc. 1961: 170.Google Scholar
  3. Hunter, M. I. S. 1971. Structural aspects of the membrane and ultrastructural features of Sarcina flava and Sarcina morrhuae.—Ph. D. Thesis, St. Andrews University.Google Scholar
  4. Hunter, M. I. S. and Thirkell, D. 1971. Variation in fatty acid composition of Sarcina flava membrane lipid with the age of the bacterial culture.—J. Gen. Microbiol. 65: 115–118.Google Scholar
  5. Huston, C. K. and Albro, P. W. 1964. Lipids of Sarcina lutea. I. Fatty acid composition of the extractable lipids.—J. Bacteriol. 88: 425–432.Google Scholar
  6. Joyce, G. H., Hammond, R. K. and White, D. C. 1970. Changes in membrane lipid composition in exponentially growing Staphylococcus aureus during the shift from 37° to 25°.—J. Bacteriol 104: 323–330.Google Scholar
  7. Kaneda, T. 1971. Factors affecting the relative ratio of fatty acids in Bacillus cereus.—Can. J. Microbiol. 17: 269–275.Google Scholar
  8. Kates, M. 1964. Bacterial lipids.—Adv. Lipid Res. 2: 17–90.Google Scholar
  9. Knivett, V. A. and Cullen, J. 1965. Some factors affecting cyclopropane acid formation in E. coli.—Biochem. J. 96: 771–776.Google Scholar
  10. Law, J. H., Zalkin, H. and Kaneshiro, T. 1963. Transmethylation reactions in bacterial lipids.—Biochim. Biophys. Acta 70: 143–151.Google Scholar
  11. Lennarz, W. J. and Talamo, B. 1966. The chemical characterization and enzymatic synthesis of mannolipids in Micrococcus lysodeikticus.—J. Biol. Chem. 241: 2707–2719.Google Scholar
  12. Macfarlane, M. G. 1961. Composition of lipid from protoplast membranes and whole cells of Micrococcus lysodeikticus.—Biochem. J. 79: 4p-5p.Google Scholar
  13. Morrison, S. J., Tornabene, T. G. and Kloos, W. E. 1971. Natural lipids in the study of relationships of members of the Family Micrococcaceae.—J. Bacteriol. 108: 353–358.Google Scholar
  14. O'Leary, W. M. 1967. The chemistry and metabolism of microbial lipids.—The World Publishing Co., Cleveland and New York.Google Scholar
  15. Salton, M. R. J. and Freer, J. H. 1965. Composition of the membranes isolated from several Gram-positive bacteria.—Biochim. Biophys. Acta 107: 531–538.Google Scholar
  16. Shaw, N. and Baddiley, J. 1968. Structure and distribution of glycosyl diglycerides in bacteria.—Nature 217: 142–144.Google Scholar
  17. Shockman, G. D., Kolb, J. J., Bakay, B., Conover, M. J. and Toennies, G. 1963. Protoplast membrane of Streptococcus faecalis.—J. Bacteriol. 85: 168–176.Google Scholar
  18. Sweeley, C. C., Bentley, R., Makita, M. and Wells, W. W. 1963. Gas-liquid chromatography of trimethylsilyl derivatives of sugars and related substances.—J. Amer. Chem. Soc. 85: 2497–2507.Google Scholar
  19. Tornabene, T. G., Bennett, E. O. and Oró, J. 1967. Fatty acid and aliphatic hydrocarbon composition of Sarcina lutea grown in three different media.—J. Bacteriol. 94: 344–348.Google Scholar
  20. Veerkamp, J. H. 1971. Fatty acid composition of Bifidobacterium and Lactobacillus strains.—J. Bacteriol. 108: 861–867.Google Scholar
  21. Vorbeck, M. L. and Marinetti, G. V. 1965a. Intracellular distribution and characterisation of the lipids of Streptococcus faecalis (ATCC 9790).—Biochemistry 4: 296–305.Google Scholar
  22. Vorbeck, M. L. and Marinetti, G. V. 1965b. Separation of glycosyl diglycerides from phosphatides using silicic acid column chromatography.—J. Lipid Res. 6: 3–6.Google Scholar
  23. Wells, M. A. and Dittmer, J. C. 1963. The use of Sephadex for the removal of non-lipid contaminants from lipid extracts.—Biochemistry 2: 1259–1263.Google Scholar

Copyright information

© H. Veenman & Zonen B.V. 1974

Authors and Affiliations

  • D. Thirkell
    • 1
  • Elizabeth M. M. Gray
    • 1
  1. 1.Department of BiochemistryUniversity of St. AndrewsSt. Andrews, FifeScotland

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