Abstract
Triacontanol (TRIA) increases the dry weight and alters the metabolism of rice (Oryza sativa L.) seedlings within 10 min of application to either the shoots or roots. This activity is prevented if octacosanol (OCTA, C28 primary alcohol) is applied with the TRIA on the roots or shoots. Triacontanol activity is also stopped if the OCTA is applied at least 1 min before the TRIA on the opposite part of the seedling.
Triacontanol rapidly elicits a second messenger that moves rapidly throughout the plant resulting in stimulation of growth (dry-weight increase) and water uptake. Octacosanol also produces a second messenger that inhibits TRIA activity. We have named the putative secondary messengers elicited by TRIA and OCTA, TRIM and OCTAM, respectively. The water-soluble TRIM extracted from plants treated with TRIA increases the growth of rice seedlings about 50% more than extracts from untreated plants, within 24 h of application. Both OCTAM and OCTA inhibit the activity of TRIA but not of TRIM.
The TRIA messenger was isolated from rice roots within 1 min of a foliar application of TRIA. The TRIM elicited by TRIA will pass through a 4-mm column of water connecting cut rice shoots with their roots and can also be recovered from water in which cut stems of TRIA-treated plants have been immersed. Triacontanol applied to oat (Avena sativa L.) or tomato (Lycopersicon esculentum Mill.) shoots connected to rice roots by a 4-mm water column also results in the appearance of TRIM in rice roots.
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Abbreviations
- OCTA:
-
octacosanol
- OCTAM:
-
second messenger elicited by OCTA
- TAS:
-
tallow alkyl sulfate
- TRIA:
-
triacontanol
- TRIM:
-
second messenger elicited by TRIA
References
Abeles, F.B. (1973) Ethylene in plant biology. Academic Press, New York
Aharoni, N., Yang, S.F. (1983) Auxin induced ethylene production as related to auxin metabolism in leaf disks of tobacco and sugar beet. Plant Physiol. 73, 598–604
Bandurski, R.S., Schulze, A., Dayanandan, P., Kaufman, P.B. (1984) Response to gravity by Zea mays seedlings. I. Time course of the response. Plant Physiol. 74, 284–288
Biddington, N.L. (1986) The effects of mechanically-induced stress in plants—a review. Plant Growth Reg. 4, 103–123
Callow, J.A. (1984) Cellular and molecular recognition between higher plants and fungal pathogens. In: Encyclopedia of Plant Physiology, vol. 17: Cellular interactions, pp. 212–237, Linskens, H.F., Heslop-Harrison, J., eds. Springer-Verlag, Berlin, Heidelberg, New York, Toronto
Cleland, R.E. (1987) Auxin and cell elongation. In: Plant hormones and their role in plant growth and development, pp. 132–148, Davies, P.J., ed. Martinus Nijhoff, Dordrecht, Boston, Lancaster
Graham, J.S., Hall, G., Pearce, G., Ryan, C.A. (1986) Regulation of synthesis of proteinase inhibitors I and II in RNAs in leaves of wounded tomato plants. Planta 169, 399–405
Hoagland, D.R., Arnon, D.I. (1950) The water-culture method for growing plants without soil. Calif. Agric. Expt. Stn. Circ. No. 347
Houtz, R.L., Ries, S.K., Tolbert, N.E. (1985a) Effect of triacontanol on Chlamydomonas. I. Stimulation of growth and photosynthetic CO2 assimilation. Plant Physiol. 79, 357–364
Houtz, R.L., Ries, S.K., Tolbert, N.E. (1985b) Effect of triacontanol on Chlamydomonas. II. Specific activity of ribulose-bisphosphate carboxylate/oxygenase, ribulose-bisphosphate concentration, and characteristics of photorespiration. Plant Physiol. 79, 365–370
Jaffe, M.J. (1976) Thigmorphogenesis: a detailed characterization of the response of beans (Phaseolus vulgaris L.) to mechanical stimulation. Z. Pflanzenphysiol. 77, 437–453
Jones, J., Wert, V., Ries, S.K. (1979) Specificity of triacontanol as a plant growth stimulator and inhibition of its effect by other long-chain compounds. Planta 144, 277–282
Keen, N.T. (1974) Specific elicitors of plant phytoalexins production: determinants of race specificity in pathogens. Science 187, 74–75
Keen, N.T., Bruegger, B. (1979) Phytoalexins and chemicals that elicit their production in plants. In: Host plant resistance to pests (Am. Chem. Soc. Symp. Ser. vol.62) pp. 1–26, Hedin, P. ed. American Chemical. Society, Washington, D.C.
Kolattukudy, P.E., Walton, T.J.A. (1972) The biochemistry of plant cuticular lipids. Prog. Chem. 13, 121–175
Kolker, L.S. (1978) Analytical procedures for 1-triacontanol and its presence in plants and the environment M.S. thesis, Michigan State University, East Lansing
Laughlin, R.G., Munyon, R.L., Ries, S.K., Wert, V.F. (1983) Growth enhancement of plants by femtomole doses of colloidally dispersed triacontanol. Science 219, 1219–1221
Lesniak, A.P., Haug, A., Ries, S.K. (1986) Stimulation of ATPase activity in barley (Hordeum vulgare) root plasma membrane after treatment of intact tissues and cell-free extracts with triacontanol. Physiol. Plant. 68, 20–26
Little, T.M., Hills, F.J. (1978) Agricultural experimentation. John Wiley & Sons, New York, Chichester, Brisbane, Toronto
Mamat, A., Fontenot, J.F., Newsom, D.W. (1983) The effect of triacontanol on the growth and development of tobasco pepper. Hort Science 18, 247–249
Pennell, C., Gaspar, T., Greppin, H. (1985) Rapid interorgan communications in higher plants with special reference to flowering. Biol. Plant. (Praha) 27, 334–338
Ries, S.K. (1985) Regulation of plant growth with triacontanol. CRC Crit. Rev. Plant Sci. 2, 239–285
Ries, S.K., Houtz, R. (1983) Triacontanol as a plant growth regulator. Hort Science 18, 654–662
Ries, S.K., Wert, V.F. (1977) Growth responses of rice seedlings to triacontanol in light and dark. Planta 135, 77–82
Ries, S.K., Wert, V. (1982) Rapid effects of triacontanol in-vivo and in-vitro. J. Plant Growth Regul. 1, 117–127
Roblin, G. (1979) Mimosa pudica: a model for the study of the excitability in plants. Biol. Rev. 54, 135–153
Schildknecht, H. (1983) Turgorins, hormones of the endogenous daily rhythms of higher organized plants-detection, isolation, structure, synthesis and activity. Angew. Chem. Int. Edn. Engl. 22, 695–710
Schildknecht, H. (1984) Turgorins — new chemical messengers for plant behaviour. Endeavour N.S. 8, 113–117
Siboaka, T. (1969) Physiology of rapid movements in higher plants. Annu. Rev. Plant Physiol. 20, 165–184
Walker-Simons, M., Hollande-Czytoka, H., Anderson, J.K., Ryan, C.A. (1984) Wound signals in plants: a systematic plant wound signal alters plasma membrane integrity. Proc. Natl. Acad. Sci. USA 81, 3737–3741
Wallace, W., Secor, J., Schrader, L.E. (1984) Rapid accumulation of λ-amino butyric acid and alanine in soybean leaves in response to an abrupt transfer to lower temperature darkness, or mechanical manipulation. Plant Physiol. 75, 170–175
Yu, Y.B., Adams, D.O., Yang, S.F. (1979) Regulation of auxin induced ethylene production in mung bean hypocotyls. Role of 1-aminocyclopropane-1-carboxylic acid. Plant Physiol. 63, 589–590
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Michigan Agricultural Experiment Station Journal Article No. 12001
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Ries, S.K., Wert, V.F. Rapid elicitation of second messengers by nanomolar doses of triacontanol and octacosanol. Planta 173, 79–87 (1988). https://doi.org/10.1007/BF00394491
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DOI: https://doi.org/10.1007/BF00394491