Skip to main content
Log in

An endogenous RNA-synthesis-promoting oligopeptide from Cucurbita pepto var. patissonina

  • Published:
Planta Aims and scope Submit manuscript

Abstract

The effect of CPPTI (Cucurbita pepo patissonina trypsin inhibitor) on the growth of Cucurbita pepo var patissonina (White bush) was examined. Plants treated with CPPTI grew faster than untreated plants and similarly to those treated with gibberellic acid. Isolated cell nuclei from plants treated with CPPTI showed that of the three DNA-dependent RNA polymerases assayed, RNA polymerase I (EC2.7.7.6) activity was preferentially elevated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

CPPTI:

Cucurbita pepo patissonina trypsin inhibitor

GA3 :

gibberellic acid

IAA:

indole-3-acetic acid

kDa:

kilodalton

RP I:

DNA-dependent RNA polymerase I

References

  • Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–689

    Google Scholar 

  • Leluk, J., Otlewski, J., Wieczorek, M., Polanowski, A., Wilusz, T. (1983) Preparation and characteristics of trypsin inhibitors from the seeds of squash and zucchini. Acta Biochim. Polon. 30, 127–138

    Google Scholar 

  • Liu, S.H., Rose, K.M. (1986) Lipopolysaccharide-mediated induction of RNA polymerase I activity and amount in murine B lymphocytes. J. Biol. Chem. 261, 8520–8527

    Google Scholar 

  • Luthe, D.S., Quatrano, R.S. (1980) Transcription in isolated wheat nuclei. I. Isolated of nuclei and elimination of endogenous ribonuclease activity. Plant Physiol. 65, 305–308

    Google Scholar 

  • Rose, K.M., Stetler, D.A., Jacob, S.T. (1981) Protein kinase activity of RNA polymerase I purified from a rat hepatoma: probable function of Mr 42000 and 24600 polypeptides. Proc. Natl. Acad. Sci. USA 78, 2833–2837

    Google Scholar 

  • Szopa, J., Rose, K.M. (1986) Cleavage of the 190-kDa subunit of DNA-dependent RNA polymerase I yields small polypeptides capable of degrading DNA. J. Biol. Chem. 261, 9022–9028

    Google Scholar 

  • Szopa, J., Rzepecki, R., Wojtkiewicz, B., Szmidziński, R. (1985) An endonuclease closely associated with DNA-dependent RNA polymerase II. FEBS Lett. 189, 301–304

    Google Scholar 

  • Szopa, J., Wagner, K.G. (1984) DNA-dependent RNA polymerase II from nuclei of suspension-cultured tobacco cells. Planta 162, 132–138

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wiśniowska, J., Rudnicki, K., Leluk, J. et al. An endogenous RNA-synthesis-promoting oligopeptide from Cucurbita pepto var. patissonina . Planta 173, 46–51 (1988). https://doi.org/10.1007/BF00394486

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00394486

Key words

Navigation