Abstract
Dithiothreitol activation of spinach leaf NADP malate dehydrogenase is mediated by protein factors that have been partially purified by chromatography on DEAE cellulose. Evidence for their intrachloroplastic localization has been obtained.
Abbreviations
- DTT:
-
dithiothreitol
- MDH:
-
malate dehydrogenase
References
Bruinsma, J.: A comment on the spectrophotometric determination of chlorophyll. Biochem. Biophys. Acta 52, 576–578 (1961)
Cockburn, N., Walker, D.A., Baldry, C.W.: The isolation of Spinach chloroplasts in pyrophosphate media. Plant Physiol. 43, 1415–1418 (1968)
Jacquot, J.P., Vidal, J., Gadal, P.: Identification of a protein factor involved in dithiothreitol activation of NADP malate dehydrogenase from french bean leaves. FEBS letters 71, 223–227 (1976)
Johnson, H.S., Hatch, M.D.: Properties and regulation of leaf nicotinamide-adenine dinucleotide phosphate dehydrogenase and “malic” enzyme in plants with the C4 dicarboxylic acid pathway of photosynthesis. Biochem. J. 119, 273–280 (1970)
Leech, R.M.: The isolation of structurally intact chloroplasts. Biochim. Biophys. Acta 79, 637–639 (1964)
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
Schürmann, P., Wolosiuk, R.A., Breazeale, V.D., Buchanan, B.B.: Two proteins function in the regulation of photosynthetic CO2 assimilation in chloroplasts. Nature 263, 257–258 (1976)
Ting, I.P., Rocha, V.: NADP specific malate dehydrogenase of green Spinach leaf tissue. Arch. Biochem. Biophys. 147, 156–164 (1971)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jacquot, J.P., Vidal, J. & Gadal, P. Evidence for chloroplastic localization of spinach leaf NADP malate dehydrogenase activating factors. Planta 137, 89–90 (1977). https://doi.org/10.1007/BF00394441
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00394441