Abstract
The effects of temperature on the cell cycle of Haplopappus gracilis suspension cultures were analysed by the fraction of labelled mitoses method. Sphase in these cultures shows a different temperature optimum as compared to optima derived for G2 and mitosis. G1 phase has a much lower Q10 than the other cell cycle phases and shows no temperature optimum between 22 and 34° C. These results are discussed in relation to a transition probability model of the cell cycle proposed by Smith and Martin (Proc. Natl. Acad. Sci. USA 70, 1263–1267, 1973), in which each cell has a time independent probability of initiating the transition into another round of DNA replication and division. The implications of such a model for cell cycle analysis are discussed and a tentative model for a probabilistic transition trigger is advanced.
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Abbreviations
- FLM:
-
Fraction of labelled mitoses
- TB :
-
Total B-phase
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Gould, A.R. Temperature response of the cell cycle of Haplopappus gracilis in suspension culture and its significance to the G1 transition probability model. Planta 137, 29–36 (1977). https://doi.org/10.1007/BF00394431
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DOI: https://doi.org/10.1007/BF00394431