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Differential chromosomal radiosensitivity within the first G1-phase of the cell cycle of early-dividing human leukocytes in vitro after stimulation with PHA

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Summary

Human leukocyte cultures were irradiated with 200 R X-rays before the addition of phytohemagglutinin (PHA) in the G0-stage and at different times up to 25 h within the first G1-phase of the cell cyle after the addition of PHA. The results of the analysis of chromosomal aberrations show that the frequencies of dicentric chromosomes increase significantly when leukocytes leave the G0-stage, reaching a maximum yield of aberrations about halfway through the first G1-phase. After that, toward the end of the G1-phase, the frequencies of dicentric chromosomes decrease again, to a level similar to that found in the G0-stage. Different possible explanations for the differential chromosomal radiosensitivity of human leukocytes within the first poststimulation G1-phase are discussed.

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  1. Institut für Genetik, Freie Universität Berlin, Arnimallee 5-7, D-1000, Berlin 33

    Bernd Beek & Günter Obe

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  1. Bernd Beek
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  2. Günter Obe
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Beek, B., Obe, G. Differential chromosomal radiosensitivity within the first G1-phase of the cell cycle of early-dividing human leukocytes in vitro after stimulation with PHA. Hum Genet 35, 209–218 (1977). https://doi.org/10.1007/BF00393972

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  • DOI: https://doi.org/10.1007/BF00393972

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