Abstract
The growth kinetics and the macromolecular content of the yeast and ethanol-induced hyphal forms of Aureobasidium pullulans were studied. During the morphological transition from yeasts to hyphae, both the protein and RNA content decreased significantly, the mycelial form containing only 76% of the amount of protein in the yeasts, and 38% of the RNA. The DNA was the only component tested whose level increased during the transition. Among several compounds inhibiting macromolecular synthesis, only hydroxyurea showed a remarkable effect on the morphology of A. pullulans, inducing the mycelial morphology. The macromolecular composition of hydroxyurea-treated cultures changed with time in a way similar to that of the ethanol-Tween 80-ammonia medium, and to that of carbon-starved cultures, without ethanol or glucose.
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References
Brown, G.A. (1965) Biochemistry of morphogenesis in black yeast like fungi, Ph. D. Thesis, Rutgers State University. New Brunswick, New Jersey
Bull, A.T. & A.P.J. Trinci (1977) The physiology and metabolic control of fungal growth. Adv. Microbial. Physiol. 15: 1–84
Burton, K. (1956) A study of the condition and mechanisms of the diphenylamine reaction for the colorimetric estimation of DNA. Biochem J 62: 315–323
Dabrowa, N., D.H. Howard, J.W. Landau & Y. Shechter (1970) Synthesis of nucleic acids and proteins in the dimorphic forms of Candida albicans. Sabouraudia 8: 163–169
Dastidar, S.G. (1965) Studies on nucleic acids and protein contents of Candida albicans and their relationship to dimorphism. Indian J. Exp. Biol. 4: 51–52
De Bievre, C. (1967) Sur la composition proteique des phases levuriformes et filamenteuses de Sporotrichum schenckii, étudiée par électrophorèse en gel d'acrylamide. C.R. Acad. Sci. Paris 265: 537–539
Haidle, C.W. & R. Storck (1966) Inhibition by cycloheximide of protein and RNA synthesis in Mucor rouxii. Biochem. Biophys. Res. Commun. 22: 175–180
Knight, R.H., B.A. Body, G.S. Kobayashi & G. Medoff (1980) Balanced growth and morphogenesis of Histoplasma capsulatum in a defined medium. Sabouraudia 18: 39–50
Lowry, D.H., N.J. Rosebrough, A.L. Farr & R.J. Randall (1951) Proteins measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275
Mariat, F. (1960) Sur la composition en acides nucléiques de la phase mycélienne de Sporotichum schenckii. C.R. Acad. Sci. Paris 250: 3368–3370
McGowan, K.L. & H.R. Buckley (1984) Growth characteristics of the yeast phase of Paracoccidioides brasiliensis in a chemically defined medium. J. Gen. Microbiol. 130: 2797–2801
Ogur, M. & G. Rosen (1950) Archives of Biochemistry 25: 262–276. In: J.R. Norris & D.W. Ribbons (Eds) Methods in Microbiology. Vol. 5B (pp. 313). London, Academic Press, 1971
Odds, F.C. (1985) Morphogenesis in Candida albicans. CRC Crit. Rev. Microbiol. 12: 45–93
Park, D. (1984) Population density and yeast mycelial dimorphism in Aureobasidium pullulans. Trans. Br. Mycol. Soc. 82: 39–44
Ramos, S. & I. Garcia-Acha (1975) A vegetative cycle of Pullularia pullulans. Trans Br Mycol Soc 64: 129–135
Roberts, R.L., R.J. Lo & P.J. Szaniszlo (1980) Induction of synchronous growth in the yeast phase of Wangiella dermatitidis. J. Bacteriol. 141: 981–984
Schneider, W.C. (1945) Determination of nucleic acids in tissues by pentose analysis. In: S.P. Collowic & N.O. Kaplan (Eds) Methods in Enzymology. Vol. III (pp. 680–684). London & New York, Academic Press, 1957
Schulz, B.E., G. Kraepelin & W. Hinkelmann (1974) Factors affecting dimorphism in Mycotypha (Mucorales): a correlation with the fermentation respiration equilibrium. J. Gen. Microbiol. 82: 1–13
Sevilla, M.J., P. Isusi, R. Gutierrez, L. Egea & F. Uruburu (1977) Influence of carbon and nitrogen sources on the morphology of Pullularia pullulans. Trans. Br. Mycol. Soc. 68: 300–303
Sevilla, M.J., L. Landajuela & F. Uruburu (1983) The effects of alcohol on the morphology of Aureobasidium pullulans. Curr. Microbiol. 9: 169–172
Shepherd, M.G., Y.Y. Chiew, S.P. Ram & P.A. Sullivan (1980) Germ tube induction in Candida albicans. Can. J. Microbiol. 26: 21–26
Slater, M.L. (1973) Effects of reversible inhibition of deoxyribonucleic acid synthesis on the yeast cell cycle. J. Bacteriol. 113: 263–270
Soll, D.R. (1985) The cell cycle and commitment to alternate cell fates in Candida albicans. In: P. Nurse & E. Streiblova (Eds) The Microbial Cell Cycle. Boca Raton, Florida, C.R.C. Press
Staebell, M. & D.R. Soll (1985) Temporal and spatial differences in cell wall expansion during bud and mycelium formation in Candida albicans J. Gen. Microbiol. 131: 1467–1480
Taylor, J.J. (1961) Nucleic acids and dimorphism in Blastomyces. Exp. Cell. Res. 24: 155–158
Yamaguchi, H. (1974a) Dimorphism in Candida albicans. I. Morphology-dependent changes in cellular content of macromolecules and respiratory activity. J. Gen. Appl. Microbiol. 20: 87–99
Yamaguchi, H. (1974b) Mycelial development and chemical alteration of Candida albicans from biotin insufficiency. Sabouraudia 12: 320–328
Yamaguchi, H. (1975) Control of dimorphism in Candida albicans by Zinc: effect on cell morphology and composition. J. Gen. Microbiol. 86: 370–372
Zorzopulos, J., A.J. Jolbagy & H.F. Terenzi (1973) Effects of ethylendiaminotetraacetate and chloranfenicol on mitochondrial activity and morphogenesis in Mucor rouxii. J. Bacteriol. 115: 1198–1204
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Sevilla, M.J., Moragues, M.D. & Uruburu, F. Growth and macromolecular content of the dimorphic fungus Aureobasidium pullulans and the effect of hydroxyurea and other inhibitors. Antonie van Leeuwenhoek 54, 75–84 (1988). https://doi.org/10.1007/BF00393959
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DOI: https://doi.org/10.1007/BF00393959