Summary
We have cloned the entire mitochondrial genome of Locusta migratoria in four fragments and characterised by restriction mapping. In addition, we have sequenced a 1,095 kb region containing the ND-1 (URF-1) gene. The inferred primary structure of the protein is highly homologous to its Drosophila counterpart (68%). The gene is flanked at the 5′ end by the tRNA leuCUN gene, interrupted by the sequence TTG. The 3′ end is flanked by the tRNA UCNser gene, followed by a sequence homologous to the 3′ end of D. yakuba cytochrome b. The relative position of the genes is conserved between Locusta and Drosophila, thus indicating conservation of mitochondrial gene order in insects.
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References
Alwine JC, Kemp DJ, Stark GR (1977) Proc Natl Acad Sci USA 74:5350–5354
Anderson S, Bankier AT, Barell BG, de Bruijn MHL, Coulson AR, Drouin J, Peron IC, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJH, Staden R, Young IG (1981) Nature 290:457–465
Anderson S, de Bruijn MHL, Coulson AR, Eperon IC, Sanger F, Young IG (1982) J Mol Biol 156:683–717
Attardi G (1985) Int Rev Cytol 93:93–145
Bibb MJ, Van Etten RA, Wright CT, Walberg MW, Clayton DA (1981) Cell 26:167–180
Brown TA, Davies RW, Ray IA, Waring RB, Scazzocchio C (1983) EMBO J 2:426–435
Chomyn A, Mariottini P, Cleeter MWJ, Ragan CI, Matsuno-Yagi A, Hatefi Y, Doolittle RF, Attardi G (1985) Nature 314:592–597
Chomyn A, Cleeter MWJ, Ragan CI, Riley M, Doolittle RF, Attardi G (1986) Science 234:614–618
Clary DO, Wolstenholme DR (1985) J Mol Evol 22:252–271
Clary DO, Wahlleithner IA, Wolstenholme DR (1984) Nucleic Acids Res 12:3747–3762
Gellissen G, Bradfield JY, White BN, Wyatt GR (1983) Nature 301:631–634
HsuChen CC, Kotin RM, Dubin DT (1984) Nucleic Acids Res 12:7771–7785
Ise W, Haiker H, Weiss H (1985) EMBO J 4:2075–2080
Kyte J, Doolittle RF (1982) J Mol Biol 157:105–132
Peñalva MA, Garcia JL (1986) Curr Genet 10:797–801
Pustell J, Kafatos FC (1986) Nucleic Acids Res 14:479–488
Rastl E, Dawid IB (1978) Cell 18:501–510
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Stern DB, Bang AT, Thompson WF (1986) Curr Genet 10:857–869
Uhlenbusch I, McCracken A, Gellissen G (1986) Curr Genet (in press)
Vieira J, Messing J (1982) Gene 19:259–268
Wong JHF, Ma DP, Wilson RK, Roe BA (1983) Nucleic Acids Res 11:4977–4995
Yanisch-Perron C, Vieira J, Messing J (1985) Gene 33:103–119
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Communicated by C. P. Hollenberg
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McCracken, A., Uhlenbusch, I. & Gellissen, G. Structure of the cloned Locusta migratoria mitochondrial genome: restriction mapping and sequence of its ND-1 (URF-1) gene. Curr Genet 11, 625–630 (1987). https://doi.org/10.1007/BF00393926
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DOI: https://doi.org/10.1007/BF00393926