Abstract
Palisade and spongy-parenchyma cells were isolated from the leaves of a number of mesomorphic dicotyledons by simply brushing the upper and lower sides, respectively, of the leaves with nylon brushes. Cross-contamination with the opposite cell type was minimal and both cell types were photosynthetically as active as leaf discs. The rates and early products of CO2 incorporation in the two cell types isolated from Zinnia elegans Jacq. plants grown in full natural light were the same, indicating that the photosynthetic physiology of the two cell types is quite similar.
Similar content being viewed by others
References
Arnon, D.I. (1949) Copper enzymes in isolated chloroplasts. Polyphenol oxidases in Beta vulgaris. Plant Physiol. 24, 1–15
Cataldo, D.A., Berlyn, G.P. (1974) An evaluation of selected physical characteristics and metabolism of enzymatically separated mesophyll cells and minor veins of tobacco. Am. J. Bot. 61, 957–963
Chellappan, K.P., Seeni, S., Gnanam, A. (1980) Photosynthetic studies with mesophyll protoplasts from Notonia grandiflora, a crassulacean acid metabolism plant. Physiol. Plant. 48, 403–410
Frederick, S.E., Newcomb, E.H. (1969) Microbody-like organelles in leaf cells. Science 163, 1353–1355
Gnanam, A., Kulandaivelu, G. (1969) Photosynthetic studies with leaf cell suspensions from higher plants. Plant Physiol. 44, 1451–1456
Haberlandt, G. (1914) Physiological plant anatomy translated. Today and Tomorrow's Book Agency, New Delhi
Hanson, H.C. (1917) Leaf structure as related to environment. Am. J. Bot. 4, 533–560
Jensen, R.G. (1979) The isolation of intact leaf cells, protoplasts and chloroplasts. In: Encyclopedia of plant physiology, N.S., vol. 6: Photosynthesis II, pp. 31–40, Gibbs, M., Latzko, E., eds. Springer, Berlin Heidelberg New York
Jensen, R.G., Francki, R.I.B., Zaitlin, M. (1971) Metabolism of separated leaf cells. I. Preparation of photosynthetically active cells from tobacco. Plant Physiol. 48, 9–13
Kanai, R., Edwards, G.E. (1973) Purification of enzymatically isolated mesophyll protoplasts from C3, C4 and Crassulacean acid metabolism plants using an aqueous dextran-polyethylene glycol two-phase system. Plant Physiol. 52, 484–490
Kulandaivelu, G., Gnanam, A. (1974) Physiological studies with isolated leaf cells. Early products of photosynthesis and their metabolic interconversions. Plant Physiol. 48, 9–13
Moss, D.N., Rasmussen, H.P. (1969) Cellular localization of CO2 fixation and translocation of metabolites. Plant Physiol. 44, 1063–1068
Outlaw, W.H., Jr., Fisher, D.B. (1975) Compartmentation in Vicia faba leaves. III. Photosynthesis in the palisade parenchyma and spongy parenchyma. Aust. J. Plant Physiol. 2, 435–439
Outlaw, W.H., Jr., Schmuck, C.L., Tolbert, N.E. (1976) Photosynthetic carbon metabolism in the palisade parenchyma and spongy parenchyma of Vicia faba L. Plant Physiol. 58, 186–189
Paul, J.S., Bassham, J.A. (1977) Maintenance of high photosynthetic rates in mesophyll cells isolated from Papaver somniferum. Plant Physiol. 60, 775–778
Rouhani, I., Vines, H.M., Black, C.C., Jr. (1973) Isolation of mesophyll cells from Sedum telephium leaves. Plant Physiol. 51, 97–103
Seeni, S., Gnanam, A. (1980) Photosynthesis in cell suspension cultures of the CAM plant Chamaecereus sylvestrii (Cactaceae). Physiol. Plant. 49, 465–472
Servaites, J.C., Ogren, W.L. (1977) Rapid isolation of mesophyll cells from leaves of soybean for photosynthetic studies. Plant Physiol. 59, 587–590
Steer, B.T. (1972) Leaf growth parameters associated with photosynthetic capacity in expanding leaves of Capsicum frutescens L. Ann. Bot. 36, 377–384
Woo, K.C., Canvin, D.T. (1980) Effect of ammonia on photosynthetic carbon fixation in isolated spinach leaf cells. Can. J. Bot. 58, 505–510
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Seeni, S., Mariappan, T., Gopalan, G. et al. Mechanical separation of palisade and spongy-parenchyma cells from the leaves of mesomorphic dicotyledons for photosynthetic studies. Planta 157, 105–110 (1983). https://doi.org/10.1007/BF00393643
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00393643