Abstract
Changes in the rate of CO2 uptake, chlorophyll a fluorescence and contents of metabolites were measured during illumination and darkening of maize leaves. Upon illumination, the contents of aspartate and alanine declined rapidly and there were rapid increases in the contents of 3-phosphoglycerate and triose phosphates. The amounts of pyruvate and phosphoenolpyruvate increased much more slowly. Upon darkening, the levels of 3-phosphoglycerate, phosphoenolpyruvate and triose phosphates fell sharply, while the amount of pyruvate increased. It is proposed that metabolite gradients in C4 photosynthesis are built-up during induction through interchange of carbon between amino acids, metabolites of the C4 pathway and 3-phosphoglycerate and triose phosphates, since CO2 fixation during the first 5 min of photosynthesis was insufficient to account for the observed build-up of intermediates. Changes in the rates of CO2 uptake and chlorophyll a fluorescence quenching are discussed in the light of the changes in metabolites.
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References
Arnon, D.I. (1949) Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol. 24, 1–15
Badger, M.R., Sharkey, T.D., von Caemmerer, S. (1984) The relationship between steady-state gas exchange of leaves and the levels of carbon reduction cycle intermediates. Planta 160, 305–313
Bergmeyer, H.U., Bernt, E., Mollering, H., Pfleiderer, G. (1974) l-Aspartate and l-asparagine. In: Methods of enzymatic analysis, vol. IV, pp. 1696–1700, Bergmeyer, H.U., ed. Verlag Chemie, Weinheim
Bernt, E., Bergmeyer, H.U. (1974) l-Glutamate, UV assay with glutamate dehydrogenase and NAD. In: Methods of enzymatic analysis, vol. IV, pp. 1704–1708, Bergmeyer, H.U., ed. Verlag Chemie, Weinheim
Crowther, D., Leegood, R.C., Walker, D.A., Hind, G. (1983a) Energetics of photosynthesis in Zea mays. II. Studies of the flash-induced electrochromic shift and fluorescence induction in mesophyll chloroplasts. Biochim. Biophys. Acta 722, 128–136
Crowther, D., Leegood, R.C., Hind, G. (1983 b) Annual Report, ARC Research Group on Photosynthesis, University of Sheffield, pp. 24–27
Chapman, K.S.R., Hatch, M.D. (1981) Aspartate decarboxylation in bundle sheath cells of Zea mays and its possible contribution to C4 photosynthesis. Aust. J. Plant Physiol. 8, 237–248
Creach, E., Michel, J.P., Thibault, P. (1974) Aspartic acid as an internal CO2 reservoir in Zea mays: effect of oxygen concentration and of far-red illumination. Planta 118, 91–100
Furbank, R.T., Walker, D.A. (1984) Photosynthetic induction in C4 leaves: an investigation using infra-red gas analysis and chlorophyll a fluorescence. Planta (in press)
Furbank, R.T., Leegood, R.C. (1984) Carbon metabolism and gas exchange in leaves of Zea mays L. Interaction between the C3 and C4 pathways during photosynthetic induction. Planta 162, 457–462
Hatch, M.D. (1979) Mechanism of C4 photosynthesis in Chloris gayana: pool sizes and kinetics of 14CO2 incorporation into 4-carbon and 3-carbon intermediates. Arch. Biochem. Biophys. 194, 117–127
Hatch, M.D., Osmond, C.B. (1979) Compartmentation and transport in C4 photosynthesis. In: Encyclopedia of plant physiology, vol. 3: Intracellular interactions and transport processes, pp. 144–184, Stocking, C.R., Heber, U., eds. Springer, Berlin Heidelberg New York
Huber, S.C., Edwards, G.E. (1975) Regulation of oxaloacetate, aspartate, and malate formation in mesophyll protoplast extracts of three types of C4 plants. Plant Physiol. 56, 324–331
Krause, G.H. (1973) The high energy state of the thylakoid system as indicated by chlorophyll fluorescence and chloroplast shrinkage. Biochim. Biophys. Acta 292, 715–728
Krause, G.H., Vernotte, C., Briantais, J.-M. (1982) Slow photoinduced quenching of chlorophyll fluorescence in intact chloroplasts and algae. Resolution into two components. Biochim. Biophys. Acta 679, 116–124
Leegood, R.C., Walker, D.A. (1980) Autocatalysis and light activation of enzymes in relation to photosynthetic induction in wheat chloroplasts. Arch. Biochem. Biophys. 200, 575–582
Leegood, R.C., Walker, D.A. (1983) Modulation of NADP-malate dehydrogenase activity in maize mesophyll chloroplasts. Plant Physiol. 71, 513–518
Leegood, R.C., Crowther, D., Walker, D.A., Hind, G. (1983) Energetics of photosynthesis in Zea mays. I. Studies of the flash-induced electrochromic shift and fluorescence induction in bundle sheath cells. Biochim. Biophys. Acta 722, 116–126
Lowry, O., Passonneau, J.V. (1972) A flexible system of enzymatic analysis. Academic Press, New York London
Quick, W.P., Horton, P. (1984) Studies on the induction of chlorophyll fluorescence in barley protoplasts. I. Factors affecting the observation of oscillations in the yield of chlorophyll fluorescence and the rate of oxygen evolution. Proc. R. Soc. London Ser. B 220, 361–370
Shieh, Y.-J., Ku, M.S.B., Black, C.C., Jr. (1982) Photosynthetic metabolism of aspartate in mesophyll and bundle sheath cells isolated from Digitaria sanguinalis (L.) Scop., a NADP+-malic enzyme C4 plant. Plant Physiol. 69, 776–780
Stitt, M., Wirtz, W., Heldt, H.W. (1980) Metabolite levels during induction in the chloroplast and extrachloroplast compartments of spinach protoplasts. Biochim. Biophys. Acta 593, 85–102
Stitt, M., Wirtz, W., Heldt, H.W. (1983) Regulation of sucrose synthesis by cytoplasmic fructosebisphosphatase and sucrose phosphate synthesis during photosynthesis in varying light and carbon dioxide. Plant Physiol. 72, 767–774
Vernon, L.P. (1960) Spectrophotometric determination of chlorophylls and pheophytins in plant extracts. Anal. Chem. 32, 1144–1150
Vernotte, C., Etienne, A.L., Briantais, J.-M. (1979) Quenching of the system II chlorophyll fluorescence by the plastoquinone pool. Biochim. Biophys. Acta 545, 519–527
Walker, D.A. (1981) Secondary fluorescence kinetics of spinach leaves in relation to the onset of carbon assimilation. Planta 153, 273–278
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Leegood, R.C., Furbank, R.T. Carbon metabolism and gas exchange in leaves of Zea mays L.. Planta 162, 450–456 (1984). https://doi.org/10.1007/BF00393458
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DOI: https://doi.org/10.1007/BF00393458