Abstract
In the marine green alga Ulva mutabilis Føyn, large daily fluctuations in respiratory and photosynthetic rates are found after the induction of synchronous formation of gametes and zoospores. A sharp reduction in photosynthetic capacity preceeds the zooid-forming cell divisions. During the same period, samples for polyacrylamide electrophoresis show an initial increase followed by a decrease in the amount of ribulose-1,5-bisphosphate carboxylase. Some other transient protein changes were also noted. A new protein band with MW 51,400 appears in the 48-h sample and becomes a prominent constituent of gametes and zoospores; it may represent tubulin.
Similar content being viewed by others
Abbreviations
- PAGE:
-
polyacrylamide gel electrophoresis
- SDS:
-
sodium dodecylsulphate
References
Britz, S.J., Briggs, W.R. (1976) Cireadian rhythms of chloroplast orientation and photosynthetic capacity in Ulva. Plant Physiol. 58, 22–27
Bryan, J., Wilson, L. (1971) Are cytoplasmic microtubules heteropolymeres? Proc. Natl. Acad. Sci. USA 68, 1762–1766
Friedmann, K.L., Egel, R. (1978) Protein patterns during sporulation in fission yeast. Z. Naturforsch. 33c, 84–91
Føyn, B. (1958) Über die Sexualität und den Generationswechsel von Ulva mutabilis (N.S.). Arch. Protistenkd. 102, 473–480
Føyn, B. (1962a) Two linked autosomal genes in Ulva mutabilis. Bot. Mar. 4, 156–162
Føyn, B. (1962b) Diploid gametes in Ulva. Nature (London) 193, 300–301
Hoxmark, R.C. (1975) Experimental analysis of the life cycle of Ulva mutabilis. Bot. Mar. 18, 123–129
Hoxmark, R.C., Nordby, Ø. (1974) Haploid meiosis as a regular phenomenon in the life cycle of Ulva mutabilis. Hereditas 76, 239–250
Hushovd, O.T., Gulliksen, O.M., Nordby, Ø. (1982) Absence of major differences between soluble proteins from haploid gametophytes and diploid sporophytes of the green alga Ulva mutabilis Føyn. Planta 156, 89–91
Iwamura, T., Nagai, H., Ichimura, S. (1970) Improved methods of chlorophyll, protein, ribonuclei acid, and desoxyribonuclei acid in planktonic populations. Int. Rev. Ges. Hydrobiol. Hydrograph 55, 131–147
Jonsson, S. (1977) Controle expérimental de la gamétogenése chez l'Enteromorpha prolifera. Chlorophycée marine. C.R. Acad. Sci. Paris Ser. D 285, 709–711
Lowry, O.H., Rosenbrough, N.J., Farr, A.L., Randall, R.J. (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275
Løvlie, A. (1964) Genetic control of division rate and morphogenesis in Ulva mutabilis Føyn. C.R. Trav. Lab. Carslberg, Ser. Physiol. 34, 77–168
Nilsen, G., Nordby, Ø. (1975) A sporulation-inhibiting substance from vegetative thalli of the green alga Ulva mutabilis Føyn. Planta 125, 127–139
Nordby, Ø. (1974) Light microscopy of meiotic zoosporogenesis and mitotic gametogenesis in Ulva mutabilis Føyn. J. Cell Sci. 15, 443–445
Nordby, Ø. (1977) Optimal conditions for meiotic spore formation in Ulva mutabilis Føyn. Bot. Mar. 20, 19–28
Nordby, Ø., Hoxmark, R.C. (1972) Changes in cellular parameters during synchronous meiosis in Ulva mutabolis Føyn. Exp. Cell Res. 75, 321–328
Nordby, Ø., Nordby, G. (1976) Measurements of division stage durations and degree of synchrony during meiosis. Exp. Cell Res. 103, 31–38
Petersen, J.G.L., Kielland-Brandt, M.C., Nilsson-Tillgren, T. (1979) Protein patterns of yeast during sporulation. Carlsberg Res. Commun 44, 149–162
Schweizer, G. (1942) Universal-Schnellfärbmethode für Kernund chromosomenuntersuchung bei Pflanzen und Tieren, p. 33 Fischer, Jena
Trew, B.J., Friesen, J.D., Moeus, P.B. (1979) Two-dimensional protein patterns during growth and sporulation in Saccharomyces cerevisiae. J. Bacteriol. 138, 60–69
Author information
Authors and Affiliations
Additional information
To whom correspondence should be addressed
Rights and permissions
About this article
Cite this article
Gulliksen, O.M., Hushovd, O.T., Texmon, I. et al. Changes in respiration, photosynthesis and protein composition during induced synchronous formation of gametes and zoospores in Ulva mutabilis Føyn. Planta 156, 33–40 (1982). https://doi.org/10.1007/BF00393440
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00393440