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Discrimination between the red- and far-red-absorbing forms of phytochrome from Avena sativa L. by monoclonal antibodies

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Abstract

A set of rat monoclonal antibodies (ARC MAC 48 to 52 and 54 to 56), raised to phytochrome from dark-grown seedlings of Avena sativa L. was tested for the ability to discriminate between the red-absorbing (Pr) and far-red-absorbing (Pfr) forms of phytochrome by indirect enzyme-linked immunosorbent assay. MAC 50 bound more strongly to Pfr and MAC 49 and 52 showed preferential binding to Pr from extracts of dark-grown Avena seedlings; MAC 50 also bound more strongly to Pfr from brushite-purified phytochrome. The remainder of the monoclonal antibodies and a rabbit polyclonal antiphytochrome preparation did not discriminate between Pr and Pfr. The results provide evidence for conformational changes in defined regions of the phytochrome apoprotein upon photoconversion.

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Abbreviations

ELISA:

enzyme-linked immunosorbent assay

FR:

far-red light

McAb:

monoclonal antibody(ies)

PBS:

phosphate-buffered saline

Pfr:

far-red-absorbing form of phytochrome

Pr:

red-absorbing form of phytochrome

R:

red light

PMSF:

phenylmethylsulphonylfluoride

References

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Authors and Affiliations

  1. Plant Physiology Department, Glasshouse Crops Research Institute, Littlehampton, West Sussex, UK

    B. Thomas & S. E. Penn

  2. Monoclonal Antibody Centre, Agricultural Research Council, Institute of Animal Physiology, Babraham, Cambridge, UK

    G. W. Butcher & G. Galfre

Authors
  1. B. Thomas
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  2. S. E. Penn
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  3. G. W. Butcher
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  4. G. Galfre
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Thomas, B., Penn, S.E., Butcher, G.W. et al. Discrimination between the red- and far-red-absorbing forms of phytochrome from Avena sativa L. by monoclonal antibodies. Planta 160, 382–384 (1984). https://doi.org/10.1007/BF00393420

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  • DOI: https://doi.org/10.1007/BF00393420

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