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Gas chromatographic-mass spectroscopic identification and quantification of cyclic guanosine-3′: 5′-monophosphate in maize seedlings (Zea mays)

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Abstract

Gas chromatographic-mass spectroscopic evidence is presented for the presence of guanosine-3′: 5′-monophosphate (cGMP) in maize seedlings. The amount of cGMP (35–72 pmol g-1 fresh weight) was quantified as a tetra-silyl derivative using gas-chromatographic detection with reference to a silylated standard of authentic cGMP. Gas-chromatographic separation of tri-silyl adenosine-3′: 5′-monophosphate and tetra-silyl cGMP is demonstrated.

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Abbreviations

cAMP:

cyclic adenosine-3′: 5′-monophosphate

cGMP:

cyclic guanosine-3′: 5′-monophosphate

GC-MS:

gas chromatography-mass spectroscopy

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Authors and Affiliations

  1. Institut für Pharmazeutische Biologie der Universität, Schänzlestrasse 1, D-7800, Freiburg, Federal Republic of Germany

    Boris Janistyn

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  1. Boris Janistyn
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Janistyn, B. Gas chromatographic-mass spectroscopic identification and quantification of cyclic guanosine-3′: 5′-monophosphate in maize seedlings (Zea mays). Planta 159, 382–385 (1983). https://doi.org/10.1007/BF00393178

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  • DOI: https://doi.org/10.1007/BF00393178

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