Summary
Preincubation at 42o, before infection at permissive temperature by phage λ, of an Escherichia coli dnaB mutant, provokes a significant increase in survival and mutagenesis of ultraviolet irradiated phage as well as mutagenesis of untreated phage. Similarly to UV irradiation and many chemical mutagens, the inhibition of DNA synthesis by temperature shift of this dnaB mutant induces SOS repair. This work shows that replication blockage in bacterial DNA is not only mutagenic for bacterial DNA itself (Witkin, 1975) but also for normally replicating λ DNA, probably due to induction of diffusible products.
References
Bonhoeffer, F.: DNA transfer and DNA synthesis during bacterial conjugation. Z. Vererbungsl. 98, 141–149 (1966)
Buttin, G., Wright, M.: Enzymatic DNA degradation in E. coli: its relationship to synthetic processes at the chromosome level. Cold Spr. Harb. Symp. quant. Biol. 33, 259–269 (1968)
Defais, M., Caillet-Fauquet, P., Fox, M.S., Radman, M.: Induction kinetics of mutagenic DNA repair activity in E. coli following ultraviolet irradiation. Molec. gen. Genet. 148, 125–135 (1976)
Defais, M., Fauquet, P., Radman, M., Errera, M.: Ultraviolet reactivation and ultraviolet mutagenesis of λ in different genetic systems. Virology 43, 495–503 (1971)
Gudas, L.J., Pardee, A.B.: DNA synthesis inhibition and the induction of protein x in Escherichia coli. J. molec. Biol. 101, 459–477 (1976)
Inouye, M., Pardee, A.B.: Changes of membrane proteins and their relation to DNA synthesis and cell division of E. coli. J. biol. Chem. 245, 5813–5819 (1970)
Ishikawa, R.H., Kondo, S.: Indirect mutagenesis in phage lambda by ultraviolet preirradiation of host bacteria. J. molec. Biol. 97, 77–92 (1975)
Noack, P., Klaus, S.: Inactivation kinetics of lambda phage repressors in a mutant of E. coli temperature sensitive in DNA replication. Molec. gen. Genet. 115, 216–224 (1967)
Radman, M.: Phenomenology of an inductible mutagenic DNA repair pathway in Escherichia coli: SOS repair hypothesis. In: Molecular and environmental aspect of mutagenesis (Prakash et al., eds.), pp. 128–142. Springfield, Illinois: C.C. Thomas Publ. 1974
Radman, M.: SOS repair hypothesis: phenomenology of an inducible DNA repair which is accompanied by mutagenesis. In: Hanawalt, P.C., and Setlow, R.B. (eds.), Molecular mechanisms for repair of DNA, part A, pp. 355–367. New York: Plenum Press 1975
Radman, M., Caillet-Fauquet, P., Defais, M., Villani, G.: The molecular mechanism of induced mutations and an in vitro biochemical assay for mutagenesis. In: Montesano, R., Bartsch, H., and Tomatis, L.(ededs.) Screening tests in chemical carcinogenesis Lyon (IARC Scientific publications no 12), pp. 537–545, 1976
Rolfe, B., Shell, J., Becker, A., Heip, J., Onodera, K., Schell-Frederick, E.: A colicin-tolerant mutant of Escherichia coli with reduced levels of cyclic AMP and a strong bias towards λ lysogeny. Molec. gen. Genet. 120, 1–16 (1973)
Schwartz, M., Worcel, A.: Reinitiation of chromosome Replication in a thermosensitive DNA mutant of Escherichia coli. II. Synchronisation of Chromosome replication after temperature shifts. J. molec. Biol. 61, 329–342 (1971)
Villani, G., Defais, M., Spadari, S., Caillet-Fauquet, P., Boiteux, S., Radman, M.: On the molecular mechanism of mutagenesis by carcinogenic mutagens. In: Proceedings of the seventh International Congress of Photobiology, Rome, August 29 (1976) (in press)
Witkin, E.M.: Thermal enhancement of ultraviolet mutability in a dnaB uvrA derivative of Escherichia coli B/r evidence for inducible error-prone repair. In: Hanawalt, P.C., and Setlow, R.B. (eds.), Molecular mechanisms for repair of DNA, Part A, pp. 369–378. New York: Plenum Press 1975
Witkin, E.M.: Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bact. Rev. 40, 869–907 (1976)
Worcel, A.: Induction of chromosome reinitiation in a thermosensitive DNA mutant of E. coli. J. molec. Biol. 52, 371–386 (1970)
Wright, M., Wickner, S., Hurwith, J.: Studies on in vitro DNA synthesis isolation of dnaB gene product from Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 70, 3120–3124 (1973)
Author information
Authors and Affiliations
Additional information
Communicated by E. Witkin
Rights and permissions
About this article
Cite this article
Caillet-Fauquet, P., Defais, M. Kinetics of induction of error-prone repair of bacteriophage λ by temperature shift in an Escherichia coli dnaB mutat. Molec. gen. Genet. 155, 231–234 (1977). https://doi.org/10.1007/BF00393165
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00393165