Summary
A protein of molecular weight 78,000 daltons, missing in albomycin and phage ES18 resistant mutants, has been identified in the outer membrane of Salmonella typhimurium SL1027. Mutants with a tonB like resistance and overproduction of outer membrane proteins due to iron shortage were also isolated. The mutation which leads to the protein deficiency maps in the sid gene region, the mutation related to overproduction of proteins maps near trp. Although the S. typhimurium and the E. coli protein mediate translocation of the iron complex ferrichrome and the structurally analogous antibiotic albomycin through the outer membrane no cross-reactivity exists in binding the phages T5, T1 and ES18 or colicin M.
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References
Bennett, R.L., Rothfield, L.J.: Genetic and physiological regulation of intrinsic proteins of the outer membrane of Salmonella typhimurium. J. Bact. 127, 498–504 (1976)
Braun, V., Hancock, R.E.W., Hantke, K., Hartmann, A.: Functional organization of the outer membrane of Escherichia coli. Phage and colicin receptors as components of iron uptake systems. J. supramolec. Struct. 5, 37–58 (1976)
Braun, V., Schaller, K., Wolff, H.: A common receptor protein for phage T5 and colicin M in the outer membrane of Escherichia coli B. Biochim. biophys. Acta (Amst.) 323, 87–97 (1973)
Braun, V., Wolff, H.: Characterization of the receptor protein for phage T5 and colicin M in the outer membrane of Escherichia coli B. FEBS Lett. 34, 77–80 (1973)
Garen, A.: Thermodynamic and kinetic studies on the attachment of T1 bacteriophage to bacteria. Biochim. biophys. Acta (Amst.) 14, 163–172 (1954)
Graham, A.C., Stocker, B.A.D.: Genetics of sensitivity of Salmonella species to colicin M and bacteriophages T5, T1 and ES18. J. Bact. 130, 1214–1223 (1977)
Hacock, R.E.W., Braun, V.: Nature of the energy requirement for the irreversible adsorption of bacteriophages T1 and ϕ80 to Escherichia coli. J. Bact. 125, 409–415 (1976)
Hantke, K., Braun, V.: Membrane receptor dependent iron trausport in Escherichia coli. FEBS Lett. 49, 301–305 (1975)
Luckey, M., Neilands, J.B.: Iron transport in Salmonella typhimurium LT-2: Prevention by ferrichrome of adsorption of bacteriophages ES18 and ES18.h1 to a common cell envelope receptor. J. Bact. 127, 1036–1037 (1976)
Luckey, M., Pollack, J.R., Wayne, R., Ames, B.N., Neilands, J.B.: Iron uptake in Salmonella typhimurium: utilization of exogenous siderochromes as iron carriers. J. Bact. 111, 731–738 (1972)
Lugtenberg, B., Meijers, J., Peters, R., van der Hoek, P., van Alphen, L.: Electrophoretic resolution of the “major outer membrane protein” of Escherichia coli K12 into four bands. FEBS Lett. 58, 254–258 (1975)
Osborn, M.J., Gander, J.E., Parisi, E., Carson, J.: Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. J. biol. Chem. 247, 3962–3972 (1972)
Sanderson, K.E.: Linkage map of Salmonella typhimurium, edition IV. Bact. Rev. 36, 558–586 (1972)
Wayne, R., Frick, K., Neilands, J.B.: Siderophore protection against colicins M, B, V and la in Escherichia coli. J. Bact. 126, 7–12 (1976)
Wayne, R., Neilands, J.B.: Evidence for a Common Binding Site for Ferrichrome Compounds and Bacteriophage ϕ80 in the Cell Envelope of Escherichia coli. J. Bact. 121, 497–503 (1975)
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Communicated by H.G. Wittmann
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Braun, V., Hantke, K. & Stauder, W. Identification of the sid outer membrane receptor protein in Salmonella typhimurium SL1027. Molec. gen. Genet. 155, 227–229 (1977). https://doi.org/10.1007/BF00393164
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DOI: https://doi.org/10.1007/BF00393164