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Directed integration of an F′ plasmid by integrative suppression

Isolation of plaque forming lambda transduring phage for the dnaC gene

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Summary

A new approach for isolation of a plaque forming λ specialized transducing phage is described. It consists of directed transposition of an F′ plasmid into the gal region of a dnaAts galE - Escherichia coli strain by integrative suppression and deletion of the chlD region in order to shorten the distance between the marker of interest on the F′ and the prophage serving to prepare an LFT1 lysate.

An F′ danC + thr + plasmid was used here and λdthr and λddnaC phages were isolated. In addition, λpdnaC was obtained from a double lysogen for λddnaC and λb2.

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  1. Abteilung Mikrobiologie, Biozentrum der Universität Basel, Klingelbergstraße 70, CH-4056, Basel, Switzerland

    Shigeru Iida

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  1. Shigeru Iida
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Communicated by T. Yura

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Iida, S. Directed integration of an F′ plasmid by integrative suppression. Molec. gen. Genet. 155, 153–162 (1977). https://doi.org/10.1007/BF00393154

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  • DOI: https://doi.org/10.1007/BF00393154

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