Abstract
Membrane-bound β-1-3- and β-1-4-glucan synthetases of Saprolegnia are affected in vitro by the presence of nucleotides. Both enzymatic activities are inhibited by uridine nucleotides. Guanosine 5′-triphosphate and ATP reduce β-1-3-glucan synthesis but stimulate β-1-4-glucan production; they also increase V max without effect on the K m for uridine 5′-diphosphate glucose. The stimulation by ATP could be the result of an activation or stabilization of the enzymes and might have implications for cell-wall construction during hyphal growth.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- GTP:
-
guanosine 5′-triphosphate
- UDP:
-
uridine 5′-diphosphate
References
Delmer, D.P., Heiniger, U., Kolow, C. (1977) UDP glucose: glucan synthetase in developing cotton fibers. I. Kinetic and physiological properties. Plant Physiol 59, 713–718
Fèvre, M. (1979a) Glucanases, glucan synthetases and wall growth in Saprolegnia monoica. In: Fungal walls and hyphal growth, pp. 225–263, Burnett, J.H., Trinci, A.P.J., eds. Cambridge University Press, Cambridge
Fèvre, M. (1979b) Digitonin solubilization and protease stimulation of β-glucan synthetase of Saprolegnid. Z. Pflanzenphysiol. 95, 129–140
Fèvre, M. (1979c) Intracellular and cell wall associated (1–3) β glucanases of Saprolegnia. Mycopathologia 67, 89–94
Fèvre, M., Rougier, M. (1981) β 1–3 and β 1–4 glucan synthesis by membrane fractions from the fungus Saprolegnia. Planta 151, 232–241
Gooday, G.W. (1979) Chitin synthesis and differentiation in Coprinus cinereus. In: Fungal walls and hyphal growth, pp. 203–223. Burnett, J.H., Trinci, A.P.J., eds. Cambridge University Press, Cambridge
Haas, D., Blaschek, W., Koehler, H., Franz, G. (1981) In vitro formation of cell wall polysaccharides with membrane fractions from tobacco protoplasts or callus. In: Cell walls '81, pp. 119–127, Robinson, D.G., Quader, H., eds. Wiss. Verlagsgesellschaft, Stuttgart
Harrington, C.R., Douglas, L.J. (1981) Effects of nucleotides and sugar nucleoties on mannosyltransferases activity in Saccharomyces cerevisiae. J. Gen. Microbiol. 125, 407–414
Hopp, H.E., Romero, P.A., Daleo, G.R., Pont-Lezica, R. (1978) Synthesis of cellulose percursors: the involvement of lipid-linked sugars. Eur. J. Biochem. 84, 561–571
Larriba, G., Morales, M., Ruiz-Herrera, J. (1981) Biosynthesis of β glucan microfibrils by cell-free extracts from Saccharomyces cerevisiae. J. Gen. Microbiol. 124, 375–383
Machlis, L. (1953) Growth and nutrition of water molds in the subgenus Euallomyces. II. Optimal composition of the minimal medium. Am. J. Bot. 40, 449–460
Raymond, Y., Fincher, G.B., Mac lachlan, G.A. (1978) Tissue slice and particulate β glucan synthetase activities from Pisum epicotyls. Plant Physiol. 61, 938–942
Shematek, E.M., Braatz, J.A., Cabib, E. (1980) Biosynthysis of the yeast cell wall. I. Preparation and properties of β(1–3) glucan synthetases. J. Biol. Chem. 255, 888–894
Shematek, E.M., Cabib, E. (1980) Biosynthesis of the yeast cell wall II. Regulation of β(1–3) glucan synthetase by ATP and GTP. J. Biol. Chem. 255, 895–902
Tsai, C.M., Hassid, W.Z. (1973) Substrate activation of β(1–3) glucan synthetase and its effect on the structure of β-glucan obtained from UDP Glucose and particulate enzyme of oat coleoptiles. Plant Physiol. 51, 998–1001
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fèvre, M. Nucleotide effects on glucan-synthesis activities of particulate enzymes from Saprolegnia . Planta 159, 130–135 (1983). https://doi.org/10.1007/BF00392983
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00392983