Abstract
Serial thick sections of guard cells from Vicia faba L., Nicotiana tabacum L., Allium cepa L., Zea mays L. and Beta vulgaris L. were obtained systematically (600–800 nm) and viewed with the transmission electron microscope in an effort to demonstrate the presence or absence of a symplastic transport pathway within the stomatal complex. Eight to ten stomata from each species were examined, and no continuous plasmodesmata were found connecting guard cells to sister guard cells or to adjacent epidermal or subsidiary cells. Continuous plasmodesmata were observed in immature guard cells, but were sealed (truncated) during the development of the mature cell wall. Histochemical stains, phosphotungstic acid and silver methenamine, were used to demonstrate differentiation within the mature guard-cell wall. The structural differentiation of the stomatal apoplastic region is discussed in relation to fanctional specialization. Plasma-membrane elaborations or plasmalemmasomes were identified in the guard cells of Zea, and it is suggested that these structures may function in ion transport.
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Abbreviations
- PTA-HCl:
-
phosphotungstic acid and hydrochloric acid
- SM:
-
silver methenamine
- UA-LC:
-
uranyl acetate and lead citrate
References
Allaway, W.G., Setterfield, G. (1972) Ultrastructural observations on guard cells of Vicia faba and Allium porrum. Can. J. Bot. 50, 1405–1413
Brown, W.V., Johnson, Sr.C. (1962) The fine structure of the grass guard cell. Am. J. Bot. 49, 110–115
Franceschi, V.R., Lucas, W.J. (1982) The relationship of the charasome to chloride uptake in Chara corallina: physiological and histochemical investigations. Planta 154, 525–537
Fujino, M., Jinno, N. (1972) The fine structure of the guard cell of Commelina communis L. Sci. Bull. Fac. Educ., Nagasaki Univ. 23, 101–111
Galatis, B., Mitrakos, K. (1980) The ultrastructural cytology of the differentiating guard cells of Vigna sinensis. Am J. Bot. 67, 1243–1261
Hayat, M.A. (1975) Positive staining for electron microscopy. Van Nostrand Reinhold, New York
Hoagland, D.R., Arnon, D.I. (1950) The water-culture method for growing plants without soil. Calif. Agric. Exp. Stn., Circ. No. 347
Inamdar, J.A., Patel, R.C., Bhatt, D.C. (1971) Structure and development of stomata in some leptosporangiate ferns. Ann. Bot. (London) 35, 643–651
Litz, R.E., Kimmins, W.C. (1968) Plasmodesmata between guard cells and accessory cells. Can. J. Bot. 46, 1603–1604
Lucas, W.J., Franceschi, V.R. (1982) Organization of the sieveelement walls of leaf minor veins. J. Ultrastruct. Res. 81, 209–221
Palevitz, B.A., Hepler, P.K. (1976) Cellulose microfibril orientation and cell shaping in developing guard cells of Allium: the role of microtubules and ion accumulation. Planta 132, 71–93
Pallas, J.E., Jr., Mollenhauer, H.H. (1972a) Physiological implications of Vicia faba and Nicotiana tabacum guard-cell ultrastructure. Am. J. Bot. 59, 504–514
Pallas, J.E., Jr., Mollenhauer, H.H. (1972b) Electron microscopic evidence for plasmodesmata in dicotyledonous guard cells. Science 175, 1275–1276
Pease, D.C. (1966) Polysaccharides associated with the exterior surface of epithelial cells: kidney, intestine, brain. J. Ultrastruct. Res. 15, 555–588
Peterson, R.L., Hambleton, S. (1978) Guard cell ontogeny in leaf stomata of the fern Ophioglossum petiolatum. Can. J. Bot. 56, 2836–2852
Rambourg, A. (1967) An improved silver methenamine technique for the detection of periodic acid-reactive complex carbohydrates with the electron microscope. J. Histochem. Cytochem. 15, 409–412
Raschke, K. (1979) Movements of stomata. In: Encyclopedia of plant physiology, N.S., vol. 7: Physiology of movements, pp. 383–441, Haupt, W., Feinleib, M.E., eds. Springer, Berlin Heidelberg New York
Raschke, K., Fellows, M.P. (1971) Stomatal movement in Zea mays: shuttle of potassium and chloride between guard cells and subsidiary cells. Planta 101, 296–316
Reynolds, E.S. (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 17, 208–213
Robards, A.W., Kidwai, P. (1969) Vesicular involvement in differentiating plant vascular cells. New Phytol. 68, 343–349
Rutter, J.C., Willmer, C.M. (1979) A light and electron microscopy study of the epidermis of Paphiopedilum spp. with emphasis on stomatal ultrastructure. Plant Cell Environ. 2, 211–219
Sack, F., Paolillo, D.J. Jr (1983) Structure and development of walls in Funaria stomata. Am. J. Bot. 70, 1019–1030
Singh, A.P., Srivastava, L.M. (1973) The fine structure of pea stomata. Protoplasma 76, 61–82
Spurr, A.R. (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31–43
Srivastava, I.M., Singh, A.P. (1972) Stomatal structure in corn leaves. J. Ultrastruct. Res. 39, 345–363
Wangermann, E., Withers, L.A. (1978) Auxin transport characteristics and cellular ultrastructure of different types of parenchyma. New Phytol. 81, 1–17
Waterkeyn, L., Bienfait, A. (1966) Production et degradation successives de callose membranaire dans les stomates en formation, chez quelque Fougeres. C.R. Acad. Sci. Paris D 262, 251–254
Willmer, C.M., Sexton, R. (1979) Stomata and plasmodesmata. Protoplasma 100, 113–124
Ziegler, H., Shmueli, E., Lange, G. (1974) Structure and function of the stomata of Zea mays. I. The development. Cytobiologie 9, 162–168
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Wille, A.C., Lucas, W.J. Ultrastructural and histochemical studies on guard cells. Planta 160, 129–142 (1984). https://doi.org/10.1007/BF00392861
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DOI: https://doi.org/10.1007/BF00392861