Abstract
The senescence of leaves is characterized by yellowing as chlorophyll pigments are degraded. Proteins of the chloroplasts also decline during this phase of development. There exists a non-yellowing mutant genotype of Festuca pratensis Huds. which does not suffer a loss of chlorophyll during senescence. The fate of chloroplast membrane proteins was studied in mutant and wild-type plants by immune blotting and immuno-electron microscopy. Intrinsic proteins of photosystem II, exemplified by the light-harvesting chlorophyll a/b-binding protein (LHCP-2) and D1, were shown to be unusually stable in the mutant during senescence, whereas the extrinsic 33-kilodalton protein of the oxygen-evolving complex was equally lable in both genotypes. An ultrastructural study revealed that while the intrinsic proteins remained in the internal membranes of the chloroplasts, they ceased to display the heterogenous lateral distribution within the lamellae which was characteristic of nonsenescent chloroplasts. These observations are discussed in the light of possible mechanisms of protein turnover in chloroplasts.
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Abbreviations
- kDa:
-
kilodalton
- LHCP-2:
-
light-harvesting chlorophyll a/b-binding protein
- Mr :
-
relative molecular mass
- PSII:
-
photosystem II
- SDS:
-
sodium dodecyl sulphate
References
Barber, J., Marder, J.B. (1986) Photosynthesis and the application of molecular genetics. Biotechnol. Genet. Eng. Rev. 4, 355–404
Ben-David, H., Nelson, N., Gepstein, S. (1983) Differential changes in the amount of protein complexes in the chloroplast membrane senescence of oat and bean leaves. Plant Physiol. 73, 507–510
Evans, P.K., Anderson, J.M. (1986) The chlorophyll a/b proteins of PSI and PSII are immunologically related. FEBS Letts. 199, 227–233
Greenberg, B.M., Gaba, V., Mattoo, A.K., Edelman, M. (1986) Identification of a primary in vivo degradation product of the rapidly-turned-over 32 kD protein of Photosystem II. EMBO J 6, 2865–2869
Griffiths, W.T., Walker, C.J. (1986) Photoreactions in chloroplast development. In: Progress in photosynthesis research, vol. IV, pp. 469–474, J. Biggins, ed. Martinus Nijhoff, Dordrecht
Harwood, J.L., Jones, A.V.H.M., Thomas, H. (1982) Leaf senescence in a non-yellowing mutant of Festuca pratensis. III. Total acyl lipids of leaf tissue during senescence. Planta 156, 152–157
Hilditch, P.I. (1986) Immunological quantification of the chlorophyll a/b binding protein in senescing leaves of Festuca pratensis Huds. Plant Sci. 45, 95–99
Hilditch, P.I., Thomas, H., Rogers, L.J. (1986a) Leaf senescence in a non-yellowing mutant of Festuca pratensis: photosynthesis and photosynthetic electron transport. Planta 167, 146–151
Hilditch, P.I., Thomas, H., Rogers, L.J. (1986b) Two processes for the breakdown of the QB protein of chloroplasts. FEBS Letts. 208, 313–318
Ljungberg, U., Akerlund, H.-E., Larsson, C., Anderson, B. (1984) Identification of polypeptides associated with the 23 and 33 kDa proteins of photosynthetic oxygen evolution. Biochem. Biophys. Acta 767, 145–152
Miller, B.L., Huffaker, R.C. (1981) Hydrolysis of ribulose-1,5-bisphosphate carboxylase by partially purified endoproteinases of senescing barley leaves. In: Genetic engineering of symbiotic nitrogen fixation and conservation of fixed nitrogen, pp. 293–303, Lyons, J.M., Valentine, R.C., Phillips, D.A., Rains, D.W., Huffaker, R.C. eds. University of California, Davis
Nixon, P.J., Dyer, T.A., Barber, J., Hunter, C.N. (1986) The production of monospecific antibodies of D1 and D2 polypeptides of photosystem II. In: Progress in photosynthesis research vol. III, pp. 779–782, J. Biggins, ed. Martinus Nijhoff, Dordrecht
Reynolds, E.S. (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212
Roberts, D.R., Thompson, J.E., Dumbroff, E.B., Gepstein, S., Mattoo, A.K. (1987) Differential changes in the synthesis and steady-state levels of thylakoid proteins during bean leaf senescence. Plant Mol. Biol. 9, 343–354
Slovacek, R.E., Harvey, M.A. (1984) Studies of chloroplast thylakoid proteins using monoclonal antibodies. Biochem. Biophys. Res. Commun. 123, 995–1001
Stoddart, J.L., Thomas, H. (1982) Leaf senescence. In: Encyclopedia of plant phsiology N. S. vol. 14A: Nucleic acids and proteins in plants, pp. 592–636, Boulter, D., Parthier, B., eds. Springer-Verlag, Berlin
Tevini, M., Steinmüller, D. (1985) Composition and function of plastoglobuli. II. Lipid composition of leaves and plastoglobuli during beech leaf senescence. Planta 163, 91–96
Thomas, H. (1977) Ultrastructure, polypeptide composition and photochemical activity of chloroplasts during foliar senescence of a non-yellowing mutant genotype of Festuca pratensis. Planta 137, 53–60
Thomas, H. (1982a) Leaf senescence in a non-yellowing mutant of Festuca pratensis. I. Chloroplast membrane polypeptides. Planta 154, 212–218
Thomas, H. (1982b) Leaf senescence in a non-yellowing mutant of Festuca pratensis. II. Proteolytic degradation of thylakoid and stroma polypeptides. Planta 154, 219–223
Thomas, H. (1987) Sid: a Mendelian locus controlling thylakoid membrane disassembly in senescing leaves of Festuca pratensis. Theor. Appl. Genet. 73, 551–555
Thomas, H., Huffaker, R.C. (1981) Hydrolysis of radioactively labelled ribulose-1,5-bisphosphate carboxylase by an endopeptidase from the primary leaf of barley seedlings. Plant Sci. Lett. 20, 251–262
Thomas, H., Stoddart, J.L. (1975) Separation of chlorophyll degradation from other senescence processes in leaves of a mutant genotype of meadow fescue (Festuca pratensis). Plant Physiol. 56, 438–441
Timko, M.P., Kausch, A.P., Hand, J.M., Cashmore, A.R., Herrera-Estrella, L., van den Broeck, G., van Montagu, M. (1985) Structure and expression of nuclear genes encoding polypeptides of the photosynthetic apparatus. In: Molecular biology of the photosynthetic apparatus, pp. 381–396, Steinback, K.E., Bonitz, S., Arntzen, C.J., Bogorad, L., eds. Cold Spring Harbor Laboratory
Wells, B. (1985) Low temperature box and tissue handing device for embedding biological tissue for immunostaining in electron microscopy. Micron Microsc. Acta. 16, 49–53
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Hilditch, P.I., Thomas, H., Thomas, B.J. et al. Leaf senescence in a non-yellowing mutant of Festuca pratensis: Proteins of photosystem II. Planta 177, 265–272 (1989). https://doi.org/10.1007/BF00392815
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DOI: https://doi.org/10.1007/BF00392815