Abstract
Embryo DNA, isolated from ungerminated seeds of Lupinus angustifolius L., contains an exceptionally high amount of guanine-cytosine-rich satellite DNA. The thermal denaturation curve of total embryo DNA is biphasic with an inflexion point at 62% denaturation, indicating the presence of satellite DNA. The satellite fraction could be separated from the mainband DNA by three successive preparative CsCl-gradient centrifugations. The densities of the DNA fractions are 1.7045 g cm-3 and 1.6925 g cm-3, respectively. The percentages of guanine-cytosine calculated from these densities are comparable to the percentages of GC calculated from the melting temperatures. Finally, ressociation studies prove that foldback DNA and highly repeated sequences are much more frequent in the satellite DNA fraction than in the mainband DNA.
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Abbreviations
- C o t :
-
the product of the DNA concentration (mol nucleotides l-1) and the time (s) of incubation in a DNA reassociation reaction
- GC:
-
guanine-cytosine
- np:
-
nucleotide parirs
- ΔT :
-
temperature interval between 16 and 84% denaturation
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Strubbe, K., Van Oostveldt, P., Broekaert, D. et al. A high amount of satellite DNA in the genome of Lupinus angustifolius L.. Planta 155, 238–243 (1982). https://doi.org/10.1007/BF00392722
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DOI: https://doi.org/10.1007/BF00392722