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Marine Biology

, Volume 75, Issue 1, pp 9–17 | Cite as

Examination of hydroxamate-siderophore production by neritic eukaryotic marine phytoplankton

  • C. G. Trick
  • R. J. Andersen
  • N. M. Price
  • A. Gillam
  • P. J. Harrison
Article

Abstract

Species of neritic eukaryotic marine phytoplankton were investigated during 1982 for hydroxamate-type siderophore production under iron-sufficient and iron-deficient culture conditions. Three of the 5 Prorocentrum species examined produced siderophores. Prorocentrin, the extracellular hydroxamate-type siderophore isolated from P. minimum, was also produced by P. mariae-lebouriae and P. gracile. P. maximum and P. micans grew poorly in iron-deficient medium and did not produce intracellular or extracellular hydroxamate-type siderophores. Thalassiosira pseudonana and Dunaliella tertiolecta produced extracellular siderophores under iron-deficient conditions, but siderophore production was not detected in the other two species, Skeletonema costatum and Olisthodiscus luteus. Each species which produced extracellular Csaky-positive hydroxamate showed a similar pattern of production. Under iron-sufficient conditions there was no measurable siderophore found either intracellularly or extracellularly. Under iron-deficient culture conditions hydroxamate-type siderophore was produced 1 to 2 d after the cessation of growth in the stationary phase. Production was over a short period of time (1 to 2 d) and the siderophore did not remain in the medium. The rate of siderophore disappearance from the medium was similar to the rate of production. Each species which produced siderophores showed an increase in in vivo fluorescence coincidental with the disappearance of the extracellular siderophore from the culture medium. There was no corresponding increase in in vivo fluorescence in iron-sufficient cultures. It is suggested that in vivo fluorescence may be used as a screening procedure for determining hydroxamate-type siderophore production in eukaryotic phytoplankton. An hypothesis on the iron uptake mechanism is proposed.

Keywords

Phytoplankton Stationary Phase Iron Uptake Dunaliella Uptake Mechanism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • C. G. Trick
    • 1
  • R. J. Andersen
    • 2
    • 3
  • N. M. Price
    • 4
    • 5
  • A. Gillam
    • 1
  • P. J. Harrison
    • 4
    • 5
  1. 1.Department of OceanographyUniversity of British ColumbiaVancouverCanada
  2. 2.Department of ChemistryUniversity of British ColumbiaVancouverCanada
  3. 3.Department of OceanographyUniversity of British ColumbiaVancouverCanada
  4. 4.Department of BotanyUniversity of British ColumbiaVancouverCanada
  5. 5.Department of OceanographyUniversity of British ColumbiaVancouverCanada

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