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Phosphate sequestration by glycerol and its effects on photosynthetic carbon assimilation by leaves

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Abstract

Glycerol induced a limitation on photosynthetic carbon assimilation by phosphate when supplied to leaves of barley (Hordeum vulgare L.) and spinach (Spinacia oleracea L.). This limitation by phosphate was evidenced by (i) reversibility of the inhibition of photosynthesis by glycerol by feeding orthophosphate (ii) a decrease in light-saturated rates of photosynthesis and saturation at a lower irradiance, (iii) the promotion of oscillations in photosynthetic CO2 assimilation and in chlorophyll fluorescence, (iv) decreases in the pools of hexose monophosphates and triose phosphates and increases in the ratio of glycerate-3-phosphate to triose phosphate, (v) decreased photochemical quenching of chlorophyll fluorescence, and increased non-photochemical quenching, specifically of the component which relaxed rapidly, indicating that thylakoid energisation had increased. In barley there was a massive accumulation of glycerol-3-phosphate and an increase in the period of the oscillations, but in spinach the accumulation of glycerol-3-phosphate was comparatively slight. The mechanism(s) by which glycerol feeding affects photosynthetic carbon assimilation are discussed in the light of these results.

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Abbreviations

Chl:

chlorophyll

C i :

intercellular concentration of CO2

P:

phosphate

PGA:

glycerate-3-phosphate

Pi:

orthophosphate

triose-P:

sum of glyceraldehyde-3-phosphate and dihydroxyacetone phosphate

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Authors and Affiliations

  1. Research Institute for Photosynthesis and Department of Plant Sciences, University of Sheffield, S10 2TN, Sheffield, UK

    Richard C. Leegood & Carlos A. Labate

  2. USDA-ARS, Crop Science Department, North Carolina State University, 3127 Ligon Road, 27695, Raleigh, NC, USA

    Steven C. Huber

  3. Lehrstuhl für Pflanzenphysiologie, Universität Bayreuth, Universitätsstrasse 30, D-8580, Bayreuth, Federal Republic of Germany

    H. Ekkehard Neuhaus & Mark Stitt

Authors
  1. Richard C. Leegood
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  2. Carlos A. Labate
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  3. Steven C. Huber
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  4. H. Ekkehard Neuhaus
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  5. Mark Stitt
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Leegood, R.C., Labate, C.A., Huber, S.C. et al. Phosphate sequestration by glycerol and its effects on photosynthetic carbon assimilation by leaves. Planta 176, 117–126 (1988). https://doi.org/10.1007/BF00392487

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  • DOI: https://doi.org/10.1007/BF00392487

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