Abstract
Chloroplasts with high rates of photosynthetic O2 evolution (up to 120 μmol O2· (mg Chl)-1·h-1 compared with 130 μmol O2· (mg Chl)-1·h-1 of whole cells) were isolated from Chlamydomonas reinhardtii cells grown in high and low CO2 concentrations using autolysine-digitonin treatment. At 25° C and pH=7.8, no O2 uptake could be observed in the dark by high- and low-CO2 adapted chloroplasts. Light saturation of photosynthetic net oxygen evolution was reached at 800 μmol photons·m-2·s-1 for high- and low-CO2 adapted chloroplasts, a value which was almost identical to that observed for whole cells. Dissolved inorganic carbon (DIC) saturation of photosynthesis was reached between 200–300 μM for low-CO2 adapted chloroplasts, whereas high-CO2 adapted chloroplasts were not saturated even at 700 μM DIC. The concentrations of DIC required to reach half-saturated rates of net O2 evolution (Km(DIC)) was 31.1 and 156 μM DIC for low- and high-CO2 adapted chloroplasts, respectively. These results demonstrate that the CO2 concentration provided during growth influenced the photosynthetic characteristics at the whole cell as well as at the chloroplast level.
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Abbreviations
- Chl:
-
chlorophyll
- DIC:
-
dissolved inorganic carbon
- Km(DIC):
-
coneentration of dissolved inorganic carbon required for the rate of half maximal net O2 evolution
- PFR:
-
photon fluence rate
- SPGM:
-
silicasol-PVP-gradient medium
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Sültemeyer, D.F., Klöck, G., Kreuzberg, K. et al. Photosynthesis and apparent affinity for dissolved inorganic carbon by cells and chloroplasts of Chlamydomonas reinhardtii grown at high and low CO2 concentrations. Planta 176, 256–260 (1988). https://doi.org/10.1007/BF00392453
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DOI: https://doi.org/10.1007/BF00392453