Summary
Autolysing plant tissues are known to produce auxin when extracted with ether. It has been shown that autolysing plant, yeast and rat liver tissues produce auxin in vitro; this suggests that relatively unspecific mechanisms are involved. Furthermore, sterile plant and animal tissues which have been killed by freezing and thawing induce nodules of differentiated cells in a previously undifferentiated callus of Phaseolus vulgaris. The callus tissue is known to differentiate in response to applied gradients of auxin. Plant and animal tissues killed by boiling were considerably less effective in inducing differentiation in the tissue. The evidence indicates that auxin is a normal product of autolysing cells. It is suggested that dying cells are an important source of auxin in the plant.
Similar content being viewed by others
References
Banfield, W. M.: Effects of cane gall bacteria upon gall tissue cells of the black raspberry. Bot. Gaz. 97, 193 (1935).
Bentley, J. A., and S. Housley: Bio-assay of plant growth hormones. Physiol. Plant. 7, 405 (1954).
Blakely, L. M., and F. C. Steward: Growth induction in cultures of Haplopappus gracilis. Amer. J. Bot. 48, 351 (1961).
Bloch, R.: Wound healing in higher plants. Bot. Rev. 7, 110 (1941).
Currier, H. B.: Callose substance in plant cells. Amer. J. Bot. 44, 479 (1957).
Esau, K.: Some anatomical aspects of plant virus disease problems. Bot. Rev. 4, 548 (1938).
Gordon, S. A.: The biogenesis of auxin. Handbuch der Pflanzenphysiologie, Bd. XIV, S. 620. Berlin-Göttingen-Heidelberg: Springer 1961.
Hagen, G. L., J. E. Gunckel, and A. H. Sparrow: Morphology and histology of tumor types induced by X, gamma and beta irradiation of a tobacco hybrid. Amer. J. Bot. 48, 691 (1961).
Hemberg, T.: Über das Vorkommen wachstumhemmender Stoffe in Kartoffelknollen und die Bildung wachstumfördernder Stoffe in Wundflächen derselben. Arkiv Bot. 30 B, 7 (1943).
Jeffs, R. A., and D. H. Northcote: Experimental induced of vascular tissue in an undifferentiated plant callus. Biochem. J. 101, 146 (1966).
——: The influence of Indol-3yl acetic acid and sugar on the pattern of induced differentiation in plant tissue culture. J. Cell. Sci. 2, 77 (1967).
Jensen, W. A.: Botanical histochemistry. San Francisco and London: W. H. Fremann & Co. 1962.
Jones, L. E., A. C. Hildebrandt, A. J. Riker, and J. H. Wu: Growth of somatic tobacco cells in microculture. Amer. J. Bot. 47, 468 (1960).
Kulescha, Z.: Récherches sur l'élaboration de substances de croissance par les tissues végétaux. Rev. gén. Bot. 59, 19 (1952).
Larsen, P.: Growth substances in higher plants. Moderne Methoden der Pflanzenalyse, Bd. III, S. 565. Berlin-Göttingen-Heidelberg: Springer 1955.
Link, G. K. K., and V. Eggers: Hyperauxiny in crown gall of tomato. Bot. Gaz. 103, 87 (1941).
Nutman, P. S.: The anatomical and cytological evidence for the formation of growth promoting substances in the developing grain of rye. Ann. Bot., N.S. 3, 731 (1939).
Sheldrake, A. R., and D. H. Northcote: The production of auxin by tobacco internode tissues. New Phytol. 67, 1 (1968a).
——: The production of auxin by detached leaves. Nature (Lond.) 217, 195 (1968b).
Skoog, F., and C. O. Miller: Chemical regulation of growth and organ formation in plant tissue cultures in vitro. Symp. Soc. exp. Biol. 11, 118 (1957).
Thimann, K. V., F. Skoog, and A. C. Byers: The extraction of auxin from plant tissues. Amer. J. Bot. 29, 598 (1942).
Wetmore, R. H., and J. P. Rier: Experimental induction of vascular tissue in the callus of angiosperms. Amer. J. Bot. 50, 418 (1963).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sheldrake, A.R., Northcote, D.H. The production of auxin by autolysing tissues. Planta 80, 227–236 (1968). https://doi.org/10.1007/BF00392393
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00392393