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Comparison of de-novo flower-bud formation in a photoperiodic and a day-neutral tobacco

Planta volume 171, pages 560–564 (1987)Cite this article

Abstract

Regeneration of flower buds in thin tissue layers from pedicels of photoinduced short-day (SD) tobacco, Nicotiana tabacum L. cv. Maryland Mammoth, is described. Up to seven flower buds per explant were obtained in a medium containing Murashige and Skoog's macro- and microclements, 100 mg/l myoinositol, 0.1 mg/l thiamine-HCl, 6% glucose, 5 μM N6-benzylaminopurine, and 0.5 μM α-naphthaleneacetic acid. Usually some vegetative buds were also formed in the pedicel thin tissue layers. Thin tissue layers from other positions in the induced SD tobacco regenerated vegetative buds only. A comparative study with a day-neutral (DN) tobacco, Samsun, showed that the capacity to form de-novo flower buds was more localized and less strongly determined in photoperiodic than in the DN tobacco. The differences between the photoperiodic and DN tobaccos in flower-bud regeneration capacity are thus quantitative and not qualitative. The basis for this quantitative difference is not known, but may depend on factors controlling production of floral stimulus (florigen) and competency of cells to respond to florigen, and-or stability of the determined state to form flower buds in vitro.

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Abbreviations

BAP:

N6-benzylaminopurine

DN:

day-neutral

GA3 :

gibberellic acid

LD:

long-day

MM:

Maryland Mammoth

NAA:

α-naphthaleneacetic acid

SD:

short-day

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Affiliations

  1. MSU-DOE Plant Research Laboratory, Michigan State University, 48824-1312, East Lansing, MI, USA

    Mangalathu S. Rajeevan & Anton Lang

Authors
  1. Mangalathu S. Rajeevan
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  2. Anton Lang
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Rajeevan, M.S., Lang, A. Comparison of de-novo flower-bud formation in a photoperiodic and a day-neutral tobacco. Planta 171, 560–564 (1987). https://doi.org/10.1007/BF00392306

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  • DOI: https://doi.org/10.1007/BF00392306

Key words

  • Auxin and flower regeneration
  • Cytokinin and flower regeneration
  • Flower (determination, regeneration)
  • Nicotiana (flower regeneration)
  • Photoperiodism