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Glucose uptake by symbiotic Chlorella in the green-hydra symbiosis

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Abstract

There is a correlation between the ability of symbiotic Chlorella algae to take up glucose and their survival in green hydra grown in continuous darkness. Although normal symbionts of European green hydra, which persist at a stable level in dark-grown animals, possessed no detectable constitutive ability to take up glucose when grown in light, uptake was induced after incubation in a medium containing glucose. Further, symbionts isolated from hydra grown in darkness for two weeks had acquired a constitutive uptake ability. Neither NC64A nor 3N813A strains of algae, in artificial symbiosis with hydra, persisted in dark-grown animals, and they showed little or no uptake ability, although in culture NC64A possessed both constitutive and inducible glucose-uptake mechanisms. In contrast, mitotic indices in all three types of algae in symbiosis with hydra increased after host feeding, indicating that the factor which stimulates algal cell division is not identical to the substrate utilised during heterotrophic growth.

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Abbreviations

E/E:

normal Hydra-Chlorella symbiosis

E/NC, E/3N:

artificial symbioses between Hydra and Chlorella strains NC64A and 3N813A, respectively

3-OMG:

3-O-methyl-D-glucose

SDS:

sodium dodecyl sulfate

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  1. Department of Plant Sciences, Agricultural Science Building, University of Oxford, Parks Road, OX1 3PF, Oxford, UK

    P. J. McAuley

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McAuley, P.J. Glucose uptake by symbiotic Chlorella in the green-hydra symbiosis. Planta 168, 523–529 (1986). https://doi.org/10.1007/BF00392272

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  • DOI: https://doi.org/10.1007/BF00392272

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