Abstract
Thirty-nine antiserum preparations from eight rabbits were screened for their ability to precipitate the immunochemically distinct phytochrome that is obtained from green oat (Avena sativa L.) shoots. The antisera were obtained from rabbits immunized with either proteolytically degraded, but still photoreversible, 60-kDa (kilodalton) phytochrome, or approx. 120-kDa phytochrome, both of which were purified from etiolated oat shoots. The ability of these antisera to precipitate phytochrome from green oats was independent of the size of phytochrome used for immunization. While crude antisera immunoprecipitated as much as 80% of the phytochrome isolated from green oat shoots, antibodies immunopurified from these sera with a column of highly purified, approx. 120-kDa phytochrome from etiolated oats precipitated no more than about 5–10%.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- kDa:
-
kilodalton
- mU:
-
milliunit
References
Butler, W.L., Norris, K.H. (1960) The spectrophotometry of dense light-scattering material. Arch. Biochem. Biophys. 87, 31–40
Cordonnier, M.-M., Greppin, H., Pratt, L.H. (1986) Phytochrome from green Avena shoots characterized with a monoclonal antibody to phytochrome from etiolated Pisum shoots. Biochemistry, in press
Cordonnier, M.-M., Pratt, L.H. (1982) Immunopurification and initial characterization of dicotyledonous phytochrome. Plant Physiol. 69, 360–365
Cordonnier, M.-M., Smith, C., Greppin, H., Pratt, L.H. (1983) Production and purification of monoclonal antibodies to Pisum and Avena phytochrome. Planta 158, 369–376
Hunt, R.E., Pratt, L.H. (1979a) Phytochrome radioimmunoassay. Plant Physiol. 64, 327–331
Hunt, R.E., Pratt, L.H. (1979b) Phytochrome immunoaffinity purification. Plant Physiol. 64, 332–336
Litts, J.C., Kelly, J.M., Lagarias, J.C. (1983) Structure-function studies on phytochrome. Preliminary characterization of highly purified phytochrome from Avena sativa enriched in the 124-kilodalton species. J. Biol. Chem. 258, 11025–11031
Pratt, L.H. (1973) Comparative immunochemistry of phytochrome. Plant Physiol. 51, 203–209
Pratt, L.H. (1984a) Phytochrome purification. In: Techniques in photomorphogenesis, pp. 175–200, Smith, H., Holmes, M.G., eds. Academic Press, London New York
Pratt, L.H. (1984b) Phytochrome immunochemistry. In: Techniques in photomorphogenesis, pp. 201–226, Smith, H., Holmes, M.G., eds. Academic Press, London New York
Pratt, L.H., Wampler, J.E., Rich, E.S., Jr. (1985) An automated dual-wavelength spectrophotometer optimized for phytochrome assay. Anal. Instrument. 13, 269–287
Shimazaki, Y., Pratt, L.H. (1985) Immunochemical detection with rabbit polyclonal and mouse monoclonal antibodies of different pools of phytochrome from etiolated and green Avena shoots. Planta 164, 333–344
Sober, H.A., ed. (1970) CRC Handbook of Biochemistry: Selected data for molecular biology, 2nd edn. CRC Press, Cleveland, O. (presently, Boca Raton, Fla.), USA
Tokuhisa, J.G., Daniels, S.M., Quail, P.H. (1985) Phytochrome in green tissue: Spectral and immunochemical evidence for two distinct molecular species of phytochrome in light-grown Avena sativa L. Planta 164, 321–332
Vierstra, R.D., Quail, P.H. (1983) Purification and initial characterization of 124-kilodalton phytochrome from Avena. Biochemistry 22, 2498–2505
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shimazaki, Y., Pratt, L.H. Immunoprecipitation of phytochrome from green Avena by rabbit antisera to phytochrome from etiolated Avena . Planta 168, 512–515 (1986). https://doi.org/10.1007/BF00392270
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00392270