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Involvement of the Golgi apparatus in the secretion of α-amylase from gibberellin-treated barley aleurone cells

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Abstract

Localisation of α-amylase (EC 3.2.1.1) in barley aleurone cells treated with gibberellic acid has been achieved using protein A-gold-labelled polyclonal antibodies. Gold particles were located almost exclusively over the lumen of the rough endoplasmic reticulum and cisternae of the Golgi apparatus. The label was most concentrated over the Golgi apparatus. This indicates that the Golgi is involved in the secretion of α-amylase protein from aleurone cells.

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Abbreviations

ER:

endoplasmic reticulum

GA3 :

gibberellic acid

PBS:

phosphate-buffered saline

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Authors and Affiliations

  1. School of Botany, University of New South Wales, P.O. Box 1, 2033, Kensington, N.S.W.

    F. Gubler & A. E. Ashford

  2. CSIRO Division of Plant Industry, 2601, Canberra, A.C.T., Australia

    J. V. Jacobsen

Authors
  1. F. Gubler
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  2. J. V. Jacobsen
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  3. A. E. Ashford
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Gubler, F., Jacobsen, J.V. & Ashford, A.E. Involvement of the Golgi apparatus in the secretion of α-amylase from gibberellin-treated barley aleurone cells. Planta 168, 447–452 (1986). https://doi.org/10.1007/BF00392263

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  • DOI: https://doi.org/10.1007/BF00392263

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