Abstract
In the summer of 1983, immature embryos from 101 selfed inbred lines and germplasm stocks of Zea mays L. were examined for their ability to produce callus cultures capable of plant regeneration (regenerable cultures) using a medium with which some limited success had previously been obtained. Forty-nine of the genotypes (49%) produced callus which visually appeared similar to callus previously cultured and shown to be capable of plant regeneration. After five months, 38 of these genotypes were alive in culture and plants were subsequently regenerated from 35 (92%) of them. No correlation was observed between plant regeneration and callus growth rate, the vivipary mutation (genes vp1, 2, 5, 7, 8 and 9), or published vigor ratings based on K+ uptake by roots. When F1 hybrid embryos were cultured, 97% of the hybrids having at least one regenerable parent also produced callus capable of plant regeneration. No regenerable cultures were obtained from any hybrid lacking a parent capable of producing a regenerable callus culture.
In the summer of 1984, immature embryos from 218 additional inbred lines and germplasm stocks were plated and examined for their ability to produce regenerable callus cultures on media containing altered micronutrient concentrations, 3,6-dichloro-o-anisic acid (dicamba), glucose, and elevated levels of vitamin-free casamino acids and thiamine. Of these genotypes 199 (91%) produced callus that was regenerable in appearance. In the 1984 study, plant regeneration was noted in many commercially important inbreds, including B73, Mo17, B84, A632, A634, Ms71, W117, H993H95 and Cm105. Thus tissue-culture techniques are now available to obtain callus cultures capable of plant regeneration from immature embryos of most maize genotypes.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- dicamba:
-
3,6-dichloro-o-anisic acid
References
Alexander, D.E., Spencer, I. (1982) Registration of South African Photoperiod Insensitive Maize Composite I, II, and (Reg. No. GP 90 to GP 92). Crop. Sci. 22, 158
Armstrong, C.L., Green, C.E. (1982) Initiation of friable, embryogenic maize callus: the role of L-proline. In: Agron. Abstr., 74th Ann. Meet., p. 89, American Society of Agronomy
Cacco, G., Saccomani, M., Ferrari, G. (1983) Changes in the uptake and assimilation efficiency for sulfate and nitrate in maize hybrids selected during the period 1930 through 1975. Physiol. Plant. 58, 171–174
Chourey, P.S., Zurawski, D.B. (1981) Callus formation from protoplasts of a maize cell culture. Theor. Appl. Genet. 59, 341–344
Chu, C.C., Wang, C.C., Sun, C.S., Hsu, C., Yin, K.C., Chu, C.Y. (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci. Sin. 16, 659–688
Conger, B.V., Hanning, G.E., Gray, D.J., McDaniel, J.K. (1983) Direct embryogenesis from mesophyll cells of orchardgrass. Science 221, 850–851
Crafts, A.S. (1964) Herbicide behavior in the plant. In: The physiology and biochemistry of herbicides, pp. 75–110, Audus, L.J., ed. Academic Press, New York London
Earle, E.D. (1983) Plant regeneration from cultures of inbred W132BN in N, C and S cytoplasm. Maize Genet. Coop. Newslett. 57, 53
Fong, F., Smith, J.D., Koehler, D.E. (1983) Early events in maize seed development. 1-Methyl-3-phenyl-5-([trifluoromethyl]phenyl)-4-(IH)-pyridinone induction of vivipary. Plant Physiol. 73, 899–901
Freeling, M., Woodman, J.C., Cheng, D.S.K. (1976) Developmental potentials of maize tissue cultures. Maydica 21, 97–112
Frick, H., Bauman, L.F. (1978) Heterosis in maize as measured by K uptake properties of seedling roots. Crop Sci. 18, 99–103
Gamborg, O.L., Miller, R.A., Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151–158
Green, C.E. (1977) Prospects for crop improvement in the field of cell culture. Hort. Sci. 12, 131–134
Green, C.E. (1981) Tissue culture in grasses and cereals. In: Genetic engineering for crop improvement, pp. 107–122, Rachie, K.O., Lyman, J.M., eds. Rockefeller Foundation Working Paper, New York, USA
Green, C.E. (1982) Somatic embryogenesis and plant regeneration from the friable callus of Zea mays. In: Plant tissue culture 1982 (Proc. V. Int. Congr. Plant Tissue and Cell Culture), pp. 107–108, Fujiwara, A., ed. Japanese Association for Plant Tissue Culture, Tokyo, Japan.
Green, C.E., Phillipps, R.L. (1975) Plant regeneration from tissue culture of maize. Crop Sci. 15, 417–421
Harms, C.T., Lorz, H., Potrykus, I. (1976) Regeneration of plantlets from callus culture of Zea mays L. Z. Pflanzenzücht. 77, 347–351
Henderson, C.B. (1980) Maize research and breeders manual, No. IX: Inbreds, breeding stocks maize investigations and academic research personnel. Illinois Foundation Seeds, Champaign, Ill., USA
Hibberd, K.A. (1984) Induction, selection and characterization of mutants in maize cell cultures. In: Cell culture and somatic cell genetics of plants, pp. 571–576, Vasil, I.K., ed. Academic Press, New York London
Hoagland, D.R., Arnon, D.I. (1950) The water-culture method for growing plants without soil. Circ. No. 347, California Agricultural Experiment Station
Horn, M.E., Sherrard, J.H., Widholm, J.M. (1983) Photoautotrophic growth of soybean cells in suspension culture. Plant Physiol. 72, 426–429
Jacobson, L. (1951) Maintenance of iron supply in nutrient solutions by a single addition of ferric potassium ethylenediamine tetra-acetate. Plant Physiol. 26, 411–413
Kauffmann, K.D., Dudley, J.W. (1979) Selection Indices for Corn Grain Yield, Percent Protein, and Kernel Weight. Crop Sci. 19, 583–588
Lu, C., Vasil, I.K., Ozias-Akins, P. (1982) Somatic embryogenesis in Zea mays L. Theor. Appl. Genet. 62, 109–112
Lu, C., Vasil, V., Vasil, I.K. (1983) Improved efficiency of somatic embryogenesis and plant regeneration in tissue cultures of maize (Zea mays L.). Theor. Appl. Genet. 66, 285–289
Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497
Neuffer, M.G., Jones, L., Zuber, M.S. (1968) The mutants of maize. Crop Science Society of America, Madison, Wis., USA
Rhodes, C.A., Green, C.E., Phillips, R.L. (1982) Regenerable maize tissue cultures derived from immature tassels. Maize Genet. Coop. Newslett. 56, 148–149
Rice, T.B. (1982) Tissue culture induced genetic variation in regenerated maize inbreds. In: Proc. 37th Ann. Corn and Sorghum Industry Res. Conf., pp. 148–162, Loden, H.D., Wilkinson, D., eds. American Seed Trade Association, Washington, D.C., USA
Rice, T.B., Reid, R.K., Gordon, P.N. (1978) Morphogenesis in field crops. In: Propagation of higher plants through tissue cultre, pp. 262–277, Hughes, K.W., Henke, R., Constantin, M., eds. National Technical Information Service, U.S. Department of Commerce, Springfield, Va., USA
Sachs, M.M., Lorz, H., Dennis, E.S., Elizur, A., Ferl, R.J., Gerlach, W.L., Pryor, A.J., Peacock, W.J. (1982) Molecular genetic analysis of the maize anaerobic response. In: Maize for biological research, pp. 139–144, Sheridan, W., ed. Plant Molecular Biology Association, Charlottesville, Va., USA
Springer, W.D., Green, C.E., Kohn, K.A. (1979) A histological examination of tissue culture initiation from immature embryos of maize. Protoplasma 101, 269–281
Torne, J.M., Santos, M.A., Pons, A., Blanco, M. (1980) Regeneration of plants from mesocotyl tissue cultures of immature embryos of Zea mays L. Plant Sci. Lett. 17, 339–344
Zuber, M.S., Darrah, L.L. (1980) 1979 U.S. Corn Germplasm Base. In: Proc. 35th Ann. Corn and Sorghum Industry Res. Conf., pp. 234–249, Loden, H.D., Wilkinson, D., eds. American Seed Trade Association, Washington, D.C., USA
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Duncan, D.R., Williams, M.E., Zehr, B.E. et al. The production of callus capable of plant regeneration from immature embryos of numerous Zea mays genotypes. Planta 165, 322–332 (1985). https://doi.org/10.1007/BF00392228
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DOI: https://doi.org/10.1007/BF00392228