Abstract
Thylakoids isolated from peas (Pisum sativum cv. Kelvedon Wonder) and phosphorylated by incubation with ATP have been compared with non-phosphorylated thylakoids in their sensitivity to photoinhibition by exposure to illumination in vitro. Assays of the kinetics of fluorescence induction at 20° C and the fluorescence emission spectra at-196° C indicate a proportionally larger decrease in fluorescence as a result of photoinhibitory treatment of non-phosphorylated compared with phosphorylated thylakoids. It is concluded that protein phosphorylation can afford partial protection to thylakoids exposed to photoinhibitory conditions.
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Abbreviations
- DCMU:
-
3-(3,4-dichlorophenyl)-1,1-dimethylurea
- F 0 :
-
Level of chlorophyll fluorescence when photosystem 2 traps are open
- F m :
-
Level of chlorphyll fluorescence when photosystem 2 traps are closed
- P:
-
Maximum level of fluorescence reached in the absence of DCMU
- PSI (II):
-
photosystem I(II)
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Horton, P., Lee, P. Phosphorylation of chloroplast membrane proteins partially protects against photoinhibition. Planta 165, 37–42 (1985). https://doi.org/10.1007/BF00392209
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DOI: https://doi.org/10.1007/BF00392209