Summary
Endonuclease VII, the product of phage T4 gene 49, has been shown previously to resolve Holliday structures in vitro. Two different processes, genetic recombination and multiplicity reactivation are presumed to have Holliday structure intermediates. Other workers have shown that genetic recombination is reduced in a gene 49 mutant infection. However, in the present study, multiplicity reactivation of UV-irradiated ts or amber mutant phage defective in gene 49 was nearly identical to that of UV-irradiated wild-type phage T4. Thus endonuclease VII is not thought to be essential for multiplicity reactivation of phage T4.
This is a preview of subscription content, log in via an institution to check access.
References
Adams MH (1959) Bacteriophages. Interscience Publishers Inc., New York
Bernstein C (1981) Deoxyribonucleic acid repair in bacteriophage. Microbiol Rev 45:72–98
Bernstein C, Wallace SS (1983) DNA repair. In: Matthews C, Kutter E, Mosig G, Berget P, (eds) Bacteriophage T4. American Society for Microbiology, Washington DC
Bernstein H (1968) Repair and recombination in phage T4 I. Genes affecting recombination. Cold Spring Harbor Symp Quant Biol 33:325–331
Brenner S, Beckwith JR (1965) Ochre mutants, a new class of suppressible mutants. J Mol Biol 13:629–637
Dannenberg R, Mosig G (1983) Early intermediates in bacteriophage T4 DNA replication and recombination. J Virol 45:813–831
Davis KJ, Symonds N (1974) The pathway of recombination in phage T4, a genetic study. Mol Gen Genet 132:173–180
Epstein RH, Bolle A, Steinberg CM, Kellenberger E, Boy de la Tour E, Chevalley R, Edgar RS, Susman M, Denhardt GH, Lielausis A (1963) Physiological studies of conditional lethal mutants of bacteriophage T4D. Cold Spring Harbor Symp Quant Biol 28:375–394
Frankel FR, Batcheler ML, Clark CK (1971) The role of gene 49 in DNA replication and head morphogenesis in bacteriophage T4. J Mol Biol 62:439–463
Kemper B, Brown DT (1976) Function of gene 49 of bacteriophage T4 II. Analysis of intracellular development and the structure of very-fast sedimenting DNA. J Virol 18:1000–1015
Kemper B, Garabett M (1981) Studies on T4-head maturation I. Purification and characterization of gene-49 controlled endonuclease. Eur J Biochem 115:123–131
Livneh Z, Lehman IR (1982) Recombinational bypass of pyrimidine dimers promoted by the recA protein of Escherichia coli. Proc Natl Acad Sci USA 79:3171–3175
Minagawa T, Murakami A, Ryo Y, Yamagishi H (1983) Structural features of very-fast sedimenting DNA formed by gene 49 defective T4. Virol 126:183–193
Miyazaki J, Ryo Y, Minagawa T (1983) Involvement of gene 49 in recombination of bacteriophage T4. Genetics 104:1–9
Mizuuchi K, Kemper B, Hays J, Weisberg RA (1982) T4 endonuclease VII cleaves Holliday structures. Cell 29:357–365
Nonn EM, Bernstein C (1977) Multiplicity reactivation and repair of nitrous acid-induced lesions in bacteriophage T4. J Mol Biol 116:31–47
Prashad N, Hosoda J (1972) Role of genes 46 and 47 in bacteriophage T4 reproduction. J Mol Biol 70:617–635
Steinberg CM, Edgar RS (1962) A critical test of a current theory of genetic recombination in bacteriophage. Genetics 47:187–208
West SC, Howard-Flanders P (1983a) Formation and resolution of figure-8 DNA structures. Cell Biochem Supplement 7B, p. 147
West SC, Howard-Flanders P (1983b) Enzymatic formation of biparental figure-8 molecules from plasmid DNA and their resolution in E. coli. Fed Proc 42:1982
Author information
Authors and Affiliations
Additional information
Communicated by G. O'Donovan
Rights and permissions
About this article
Cite this article
Hyman, P. Gene 49 endonuclease VII is not essential for multiplicity reactivation of bacteriophage T4. Mol Gen Genet 192, 512–514 (1983). https://doi.org/10.1007/BF00392199
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00392199