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Cin1, a family of dispersed repetitive elements in Zea mays

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Summary

The Corn Insert, Cin1, is shown to be a heterogenous family of dispersed repetitive elements in the genome of Zea mays. Fourteen different recombinant clones were isolated from the maize Line C genome using the original Cin1-001 insert as a hybridization probe. These clones were characterized by heteroduplex analysis and restriction enzyme mapping as to the position and extent of the Cin1 region(s). The Cin1 related sequences are shown to vary in size up to approximately 700 bp.

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References

  • Benton WD, Davis RW (1977) Screening λgtWES recombinant clones by hybridization to single plaques in situ, Science 196:180–182

    Google Scholar 

  • Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucl Acids Res 7:1513–1523

    Google Scholar 

  • Chang ACY, Cohen SN (1978) Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol 134:1141–1156

    Google Scholar 

  • Dagert M, Ehrlich SD (1979) Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene 6:23–28

    Google Scholar 

  • Davidson EH, Posakony JW (1982) Repetitive sequence transcripts in development. Nature 297:633–635

    Google Scholar 

  • Flavell R (1980) The molecular characterization and organization of plant chromosomal DNA sequences. Ann Rev Plant Physiol 31:569–596

    Google Scholar 

  • Flavell RB, Bennett MD, Smith JB, Smith DB (1974) Genome size and the proportion of repeated nucleotide sequence DNA in plants. Biochem Genet 12:257–269

    Google Scholar 

  • Hake S, Walbot V (1980) The genome of Zea mays, its organization and homology to related grasses. Chromosoma 79:251–270

    Google Scholar 

  • Hohn B (1979) In vitro packaging of λ and cosmid DNA. In: Colowick SP, Kaplan NO (eds) Methods in Enzymology 68:299–310

  • Jelinek WR, Schmid CW (1982) Repetitive sequences in eukaryotic DNA and their expression. Ann Rev Biochem 51:813–844

    Google Scholar 

  • Leder P, Tiemeier D, Enquist L (1977) EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: The λ gt WES cloning system. Science 196:175–177

    Google Scholar 

  • Mascia PN, Rubenstein I, Phillips RL, Wang AS, Xiang LZ (1981) Localization of the 5S rRNA genes and evidence for diversity in the 5S rDNA region of maize. Gene 15:7–20

    Google Scholar 

  • Phillips RL, Kleese RA, Wang SS (1971) The nucleolus organizer region of maize (Zea mays L.) Chromosomal site of DNA complementary to ribosomal RNA. Chromosoma 36:79–88

    Google Scholar 

  • Rigby PWJ, Dieckmann M, Rhodes C, Berg P (1977) Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113:237–251

    Google Scholar 

  • Saedler H, Bonas U, Deumling B, Gupta M, Hahlbrock K, Harrison BJ, Kreuzaler F, Peterson PA, Reif J, Schwarz-Sommer Z, Shepherd N, Sommer H, Ubben D, Wienand U (1983) Transposable elements in plants. In: Chater KF, Cullis CA, Hopwood DA, Johnston AWB, Woolhouse HW (eds) Genetic Rearrangement. The Fifth John Innes Symposium. Croom Helm Ltd., London, Canberra

    Google Scholar 

  • Shepherd NS, Schwarz-Sommer Z, Wienand U, Sommer H, Deumling B, Peterson PA, Saedler H (1982) Cloning of a genomic fragment carrying the insertion element Cin1 of Zea mays. Mol Gen Genet 188:266–271

    Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517

    Google Scholar 

  • Viotti A, Abildsten D, Pogna N, Sala E, Pirotta V (1982) Multiplicity and diversity of cloned zein cDNA sequences and their chromosomal localization. EMBO J 1:53–58

    Google Scholar 

  • Wienand U, Sommer H, Schwarz Z, Shepherd N, Saedler H, Kreuzaler F, Ragg H, Fautz E, Hahlbrock K,Harrison B, Peterson PA (1982) A general method to identify plant structural genes among genomic DNA clones using transposable element induced mutations. Mol Gen Genet 187:195–201

    Google Scholar 

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Authors and Affiliations

  1. Max-Planck-Institut für Züchtungsforschung, Egelspfad, D-5000, Köln 30, Federal Republic of Germany

    Manju Gupta, Isolde Bertram, Nancy S. Shepherd & Heinz Saedler

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  1. Manju Gupta
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  2. Isolde Bertram
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  3. Nancy S. Shepherd
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  4. Heinz Saedler
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Communicated by E. Bautz

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Gupta, M., Bertram, I., Shepherd, N.S. et al. Cin1, a family of dispersed repetitive elements in Zea mays . Mol Gen Genet 192, 373–377 (1983). https://doi.org/10.1007/BF00392177

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  • DOI: https://doi.org/10.1007/BF00392177

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