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Analysis of the antimutagenic effect of cinnamaldehyde on chemically induced mutagenesis in Escherichia coli

Molecular and General Genetics MGG volume 192, pages 309–315 (1983)Cite this article

Summary

The antimutagenic effect of cinnamaldehyde on mutagenesis was investigated using ten kinds of chemical mutagen in Escherichia coli WP2s (uvrA −). In addition, the frequency of mutation induction by each mutagen in an SOS repair deficient (umuC −) strain was compared with that in a wild-type (umuC +) strain. Cinnamaldehyde greatly suppressed the umuC-dependent mutagenesis induced by 4-nitroquinoline 1-oxide (4-NQO), furylfuramide or captan. However, cinnamaldehyde was less effective against the umuC-independent mutagenesis by alkylating agents such as N-methyl-N′-nitro-N-nitrosoguanidine and ethylmethanesulfonate. On the other hand, no inhibitory effect of cinnamaldehyde was observed on prophage induction or tif-mediated filamentous growth. These results suggest that a cinnamaldehyde does not prevent the induction of the SOS functions. Despite the decrease in the number of revertants, a remarkable increase was observed in the survival of 4-NQO-treated WP2s cells after exposure to cinnamaldehyde. The reactivation of survival suggests the promotion of some DNA repair system by cinnamaldehyde. This enhancement of survival was also observed in uvrB, polA, recF or umuC mutants and less in lexA or recB, C mutants. However, it was not observed in recA mutants. Therefore, we assume that cinnamaldehyde may enhance an error-free recombinational repair system by acting on recA-enzyme activity.

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Abbreviations

AF-2:

furylfuramide

DES:

diethylsulfate

EMS:

ethylmethanesulfonate

ENNG:

N-ethyl-N′-nitro-N-nitrosoguanidine

ENU:

N-ethyl-N-nitrosourea

MNNG:

N-methyl-N′-nitro-N-nitrosoguanidine

MNU:

N-methyl-N-nitrosourea

4-NQO:

4-nitroquinoline 1-oxide

β-PL:

β-propiolactone

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Affiliations

  1. Toxicology Division, Institute of Environmental Toxicology, Suzuki-cho 2-772, Kodaira, 187, Tokyo, Japan

    Toshihiro Ohta, Kazuko Watanabe, Masaaki Moriya & Yasuhiko Shirasu

  2. Department of Induced Mutation, National Institute of Genetics, Yata, 411, Mishima, Shizuoka, Japan

    Tsuneo Kada

Authors
  1. Toshihiro Ohta
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  2. Kazuko Watanabe
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  3. Masaaki Moriya
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  4. Yasuhiko Shirasu
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  5. Tsuneo Kada
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Communicated by Ch. Auerbach

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Ohta, T., Watanabe, K., Moriya, M. et al. Analysis of the antimutagenic effect of cinnamaldehyde on chemically induced mutagenesis in Escherichia coli . Mol Gen Genet 192, 309–315 (1983). https://doi.org/10.1007/BF00392167

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  • DOI: https://doi.org/10.1007/BF00392167

Keywords

  • Repair System
  • Cinnamaldehyde
  • Captan
  • Ethylmethanesulfonate
  • Recombinational Repair