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Activation of ribulose-1,5-bisphosphate carboxylase by chloroplast metabolites in a reconstituted spinach chloroplast system

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Abstract

In a preparation of soluble components from isolated spinach (Spinecia oleracea L.) chloroplasts, the activity of ribulose-1,5-bisphosphate carboxylase (EC 4.1.1.39) is strongly increased by 6-phosphogluconate or by NADPH at pH 8.0. When the thylakoid system is added to these soluble components (reconstituted chloroplast system) plus ferredoxin, the carboxylase is even more strongly activated in the light. This “light” activation appears to be due to reduction of endogenous NADP+ by electrons from the light reactions transferred via ferredoxin, since NADPH alone can activate the purified enzyme in the dark while reduced ferredoxin does not. The regulatory properties of the enzyme in the reconstituted chloroplast system are compared with those of the isolated enzyme, and their possible physiologic significance is discussed.

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Abbreviations

Fd:

ferredoxin

PPC:

pentose phosphate cycle

6-PGluA:

6-phosphogluconate

Rib-5-P:

ribose-5-phosphate

RuBP:

ribulose-1,5-bisphosphate

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Authors and Affiliations

  1. Institut für Botanik und Mikrobiologie, Lehrstuhl für Botanik, Technische Universität, Arcisstraße 21, D-8000, München 2, Federal Republic of Germany

    Klaus J. Lendzian

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  1. Klaus J. Lendzian
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Lendzian, K.J. Activation of ribulose-1,5-bisphosphate carboxylase by chloroplast metabolites in a reconstituted spinach chloroplast system. Planta 143, 291–296 (1978). https://doi.org/10.1007/BF00392001

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  • DOI: https://doi.org/10.1007/BF00392001

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