Planta

, Volume 133, Issue 2, pp 191–196 | Cite as

Carboxylating enzymes and pathway of photosynthetic carbon assmilation in different marine algae—Evidence for the C4-pathway?

  • Bruno P. Kremer
  • Ursula Küppers
Article

Abstract

Experiments on short-term photosynthesis in H14CO3- (2–5 s) using various species of different algal classes resulted in predominant 14C-labelling (>90% of total 14C-incorporation) of phosphorylated compounds. The percentage of malate and aspartate usually accounts for distinctly less than 10% of the total 14C-labelling. These findings are consistent with data from enzymatic analyses, since 97–100% of the carboxylation capacity is due to ribulose-1.5-biphosphate carboxylase (EC 4.1.1.39) in Rhodophyceae and Chlorophyceae. Phaeophyceae are generally characterized by considerable activity of phosphoenolpyruvate carboxykinase (EC 4.1.1.32): at least 10% of carboxylation is confined to this enzyme. Similar ratios are obtained when rates of photosynthesis and of light-independent CO2-fixation are compared. Activity of phosphoenolpyruvate carboxylase (EC 4.1.1.31) could not be detected in the species investigated. The results are discussed with emphasis on the pathway of photosynthetic carbon assimilation in marine algae.

Key words

C4-pathway Dark fixation CO2 dark Marine macroalgae Photosynthesis 

Abbreviations

PEP-CK

phosphoenolpyruvate carboxykinase (EC 4.1.1.32)

PEP-C

phosphoenolpyruvate carboxylase (EC 4.1.1.31)

RubP-C

ribulose-1.5-biphosphate carboxylase (EC 4.1.1.39)

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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Bruno P. Kremer
    • 1
  • Ursula Küppers
    • 1
  1. 1.Botanisches Institut der UniversitätKöln 41Federal Republic of Germany

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