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Comparative biochemical and immunological studies of the glycine betaine synthesis pathway in diverse families of dicotyledons

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Abstract

Members of the Chenopodiaceae can accumulate high levels (>100 μmol·(g DW)-1) of glycine betaine (betaine) in leaves when salinized. Chenopodiaceae synthesize betaine by a two-step oxidation of choline (choline→betaine aldehyde→ betaine), with the second step catalyzed by betaine aldehyde dehydrogenase (BADH, EC 1.2.1.8). High betaine levels have also been reported in leaves of species from several distantly-related families of dicotyledons, raising the question of whether the same betaine-synthesis pathway is used in all cases.

Fast atom bombardment mass spectrometry showed that betaine levels of >100 μmol·(g DW)-1 are present in Lycium ferocissimum Miers (Solanaceae), Helianthus annuus L. (Asteraceae), Convolvulus arvensis L. (Convolvulaceae), and Amaranthus caudatus L. (Amaranthaceae), that salinization promotes betaine accumulation in these plants, and that they can convert supplied choline to betaine aldehyde and betaine. Nicotiana tabacum L. and Lycopersicon lycopersicum (L.) Karst. ex Farw. (Solanaceae), Lactuca sativa L. (Asteraceae) and Ipomoea purpurea L. (Convolvulaceae) also contained betaine, but at a low level (0.1–0.5 μmol·(g DW)-1. Betaine aldehyde dehydrogenase activity assays, immunotitration and immunoblotting demonstrated that the betaine-accumulating species have a BADH enzyme recognized by antibodies raised against BADH from Spinacia oleracea L. (Chenopodiaceae), and that the Mr of the BADH monomer is in all cases close to 63 000. These data indicate that the choline→betaine aldehyde→betaine pathway may have evolved by vertical descent from an early angiosperm ancestor, and might be widespread (albeit not always strongly expressed) among flowering plants. Consistent with these suggestions, Magnolia x soulangiana was found to have a low level of betaine, and to express a protein of Mr 63 000 which cross-reacted with antibodies to BADH from Spinacia oleracea.

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Abbreviations

BADH:

Betaine aldehyde dehydrogenase

DCIMS:

desorption chemical ionization mass spectrometry

FABMS:

fast atom bombardment mass spectrometry

Mr :

relative molecular mass

PAGE:

polyacrylamide gel electrophoresis

SDS:

sodium dodecyl sulfate

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. MSU-DOE Plant Research Laboratory, Michigan State University, 48824-1312, East Lansing, MI, USA

    Elizabeth A. Weretilnyk, Sebastian Bednarek, Kent F. McCue & Andrew D. Hanson

  2. Department of Horticulture, Purdue University, 47907, West Lafayette, IN, USA

    David Rhodes

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  1. Elizabeth A. Weretilnyk
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  2. Sebastian Bednarek
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  3. Kent F. McCue
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  4. David Rhodes
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  5. Andrew D. Hanson
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Weretilnyk, E.A., Bednarek, S., McCue, K.F. et al. Comparative biochemical and immunological studies of the glycine betaine synthesis pathway in diverse families of dicotyledons. Planta 178, 342–352 (1989). https://doi.org/10.1007/BF00391862

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  • DOI: https://doi.org/10.1007/BF00391862

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