Summary
Discrete pleomorphic colonies were cultured from a lymph node of a patient with Hodgkin's disease using phorbol myristate acetate in semi-solid media. A mean of 216 colonies were counted after 10 days incubation. No colonies were noted after similar incubation with pokeweed mitogen or lymphocyte conditioned medium. A gradation in morphology from small lymphoid cells to giant multinucleated cells was noted. The majority (80%) of the cultured cells bore T lymphoid suface markers. The use of phorbol myristate acetate as a stimulant for colony growth of cells from the lymph nodes of Hodgkin's disease patients would appear to be of value.
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References
Diehl V, Kirchner HH, Schaadt M, Fonatsch C, Stein H, Gerdes J, Boie C (1981) Hodgkin's disease: establishment and characterization of four in vitro cell lines. J Cancer Res Clin Oncol 101:111–124
Greenberger JS, Newberger PE, Sakakeeny M (1981) Phorbol myristate acetate stimulates macrophage differentiation and replication and alters granulopoiesis and leukemogenesis in long-term bone marrow cultures. Blood 56:368–379
Jones SE, Hambuger AW, Kim MB, Salmon SE (1979) Development of a bioassay for putative human lymphoma stem cells. Blood 53:294–303
Koeffler HP, Bar-Eli M, Territo M (1980) Phorbol diester-induced macrophage differentiation of leukemic blasts from patients with human myelogeneous leukemia. J Clin Invest 66:1101–1108
Mc Guire RA, Pretlow TG, Wareing TH, Bradley EL (1979) Hodgkin's cells and attached lymphocytes. A possible prognostic indicator in splenic tumor. Cancer 44:183–187
Radnay J, Goldman I, Rozenszajn LA (1979) Growth of human Blymphocytes colonies in vitro. Nature 278:351–353
Rozenszajn LA, Shoham D, Kalechman I (1975) Clonal proliferation of PHA-stimulated human lymphocytes in soft agar culture. Immunology 29:1041–1055
Skinnider LF, McAskill J (1980) A comparison of phorbol myristate acetate with phytohemagglutinin as a stimulator of in vitro colony growth of peripheral human leucocytes. Exp Hematol 8:477–483
Stuart RK, Hamilton JA (1980) Tumor-promoting phorbol esters stimulate hematopoietic colony formation in vitro. Science 208:402–404
Totterman TH, Nilsson K, Sundstrom C (1980) Phorbol ester-induced differentiation of chronic lymphocytic leukaemia cells. Nature 288:176–178
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This work was supported by a grant from the National Cancer Institute of Canada
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Skinnider, L.F., Maksymiuk, W. & McAskill, J. Phorbol myristate acetate induced colony growth in Hodgkin's disease. J Cancer Res Clin Oncol 105, 103–105 (1983). https://doi.org/10.1007/BF00391840
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DOI: https://doi.org/10.1007/BF00391840