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Determination of growth kinetics in a renal sarcoma of the rat by double labeling with 14C- and 3H-thymidine

  • Original Papers
  • Experimental Oncology
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Summary

An in vitro double-labeling technique for determination of kinetic growth parameters was tested; 13 renal sarcomas of rats were incubated after excision under standardized conditions with 14C- and 3H-thymidine successively. Another ten tumors were evaluated after in vivo double injection of 14C and 3H-thymidine into sarcoma-bearing animals. In both groups histoautoradiography was performed using a simple stripping-film method. Results including labeling indices, mitotic indices, length of DNA-synthesis phase and cell-production rate obtained by both methods were in excellent agreement. All values fitted those determined in earlier experiments using a continous labeling and a fraction-labeled-mitoses method in vivo. Theoretical considerations on the calculation of kinetic parameters by double labeling have been added. The experimental results found in this study represent a base for evaluation of biopsies from human tumors.

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Authors and Affiliations

  1. Dept. of Pathology, Medizinische Hochschule, Karl-Wiechert-Allee 9, D-3000, Hannover, Federal Republic of Germany

    W. Lang, S. Kienzle & A. Georgii

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  1. W. Lang
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  2. S. Kienzle
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  3. A. Georgii
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Supported by the Deutsche Forschungsgemeinschaft (DFG, grant Ge 121/16)

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Lang, W., Kienzle, S. & Georgii, A. Determination of growth kinetics in a renal sarcoma of the rat by double labeling with 14C- and 3H-thymidine. J Cancer Res Clin Oncol 105, 55–61 (1983). https://doi.org/10.1007/BF00391832

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  • DOI: https://doi.org/10.1007/BF00391832

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