Summary
Immunohistochemical staining of epidermal growth factor receptor (EGF-R) with a monoclonal antibody was performed in 43 biopsies of cervical tissue. The distribution of the receptors in normal cervical tissue differs from that of cervical intraepithelial neoplasia (CIN) and squamous cell carcinoma of the cervix. Whereas the staining reaction in normal squamous epithelium was confined to the basal and deep parabasal cell layer, in all cervical intraepithelial neoplasias, with or without human papilloma virus association, a homogeneous EGF-R staining reaction could be observed throughout the entire lesion. This means that the dysplasia cells of a CIN I-III, like the tumor cells of a squamous cell carcinoma, have a raised EGF-R content, which in the normal squamous epithelium is usually only found in the basal and deep parabasal cells that are capable of dividing. No EGF-R staining reaction could be detected in the higher, differentiated cell layers of the normal squamous epithelium of the cervix.
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Abbreviations
- EGF-R:
-
epidermal growth factor receptor
- CIN:
-
cervical intraepithelial neoplasia
References
Bauknecht T, Janz J, Johler M, Pfleiderer A (1989) The occurrence of epidermal growth factor receptors and the characterization of EGF like factors in human ovarian, endometrial, cervical and breast cancer. J Cancer Res Clin Oncol 115:193–199
Carpenter G, Cohen S (1979) Epidermal growth factors. Annu Rev Biochem 48:193–216
Cowley G, Smith J, Gusterson B, Hendler F, Ozanne B (1984) The amount of EGF receptors is elevated on squamous cell carcinomas. In: Levine A, Vande Woude G, Topp W, Watson JD (eds) The transformed phenotype, Cancer Cells, vol 1. Cold Spring Harbor Laboratory, New York, pp 5–10
Downward J, Yarden Y, Mayes E (1984) Close similarity of epidermal growth factor receptor and v-erbB oncogene protein sequences. Nature 307:521–537
Göppinger A, Birmelin G, Ikenberg H (1987) Human papillomavirus standardization and DNA cytophotometry in cervical intraepithelial neoplasia. J Reprod Med 32:609–613
Gullick W, Marsden J, Whittle N, Ward B, Bobrow L, Waterfield D (1986) Expression of epidermal growth factor receptors on human cervical, ovarian and vulvar carcinomas. Cancer Res 46:285–292
Gusterson B, Cowley G, Smith JA, Bradford O (1984) Cellular localization of tumor epidermal growth factor receptor. Cell Biol Rep 8:649–657
Hunts J, Ueda M, Ozawa S, Osahiko A, Pastan I, Shimizu N (1985) Hyperproduction and gene amplification of the epidermal growth factor receptor in squamous cell carcinomas. Jpn J Cancer Res 76:663–666
Kageyama R, Merlino G, Pastan I (1988) Epidermal growth factor receptor gene transcription. Br J Cancer 263:6329–6336
Nanney LB, McKanna JA, Stoscheck CM, Carpenter G, King LE (1984) Visualization of epidermal growth factor receptors in human epidermis. J Invest Dermatol 82:165–169
Poulsen HF, Taylor CW, Sobin LH (1975) Histology of the female genital tract. In: Int Hist Classification of Tumors No 8 WHO Geneva:26–33
Richart RM (1968) Natural history of intraepithelial neoplasia. Clin Obst Gynecol 10:748–753
Ullrich A, Coussens L, Hayflick JS, Dull TJ, Gray A, Tam AW, Lee J Yarden Y, Libermann TA, Schlessinger J, Downward J, Mayes ELV, Whittle N, Waterfield MD, Seeburg PH (1984) Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells. Nature 309:418–425
Wentz WB, Reagan JW (1959) Survival in cervical cancer with respect to cell type. Cancer 12:384–389
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Göppinger, A., Wittmaack, F.M., Wintzer, H.O. et al. Localization of human epidermal growth factor receptor in cervical intraepithelial neoplasias. J Cancer Res Clin Oncol 115, 259–263 (1989). https://doi.org/10.1007/BF00391699
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DOI: https://doi.org/10.1007/BF00391699