Summary
The effect of a combined treatment with shock waves generated by a lithotripter and Adriamycin or cisplatin was examined in cells that acutely survived exposure to shock waves and proliferated afterwards. Batches of 2 x 106 cells were exposed to the respective drug for 50 min or for 50 min plus 72 h. During the 50-min drug exposure 500 shock waves were applied at 25 kV. The growth as a percentage of the control was determined after 72 h by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cells treated with shock waves alone showed a growth inhibition as compared to control cells. For a 50-min drug exposure with Adriamycin the dose enhancement ratio did not exceed 1.3. For a 50-min drug exposure with cisplatin at concentrations of 0.5 μg/ml and 5.0 μg/ml, growth (as a percentage of the control) after combined treatment was significantly reduced as compared to cisplatin treatment alone; the dose enhancement ratio was 3.2 at 50% growth compared to the control. This indicates that shock waves can increase the susceptibility of L1210 cells to cisplatin. For a 50-min plus 72-h drug exposure no effect of an additional treatment with shock waves, as compared to chemotherapy alone, could be observed.
Similar content being viewed by others
Abbreviations
- DER:
-
dose-enhancement ratio
- MTT:
-
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide
References
Brendel W, Chaussy C, Forssmann B, Schmiedt E (1979) A new method of non-invasive destruction of renal calculi by shock waves. Br J Surg 66:907
Carmichael J, Degraff WG, Gazdar AF, Minna JD, Mitchell JB (1987 a) Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47:936–942
Carmichael J, Degraff WG, Gazdar AF, Minna JD, Mitchell JB (1987 b) Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of radiosensitivity testing. Cancer Res 47:943–946
Chaussy C (1986) Extracorporeal shock wave lithotripsy. Technical concept, experimental research, and clinical application, 2nd edn. Karger, Basel
Coleman AJ, Sauders JE, Crum LA, Dyson M (1987) Acoustic cavitation generated by an extracorporeal shockwave lithotripter. Ultrasound Med Biol 13:69–76
Crum LA (1982) Acoustic cavitation. Proc 1982 Ultrason Symp 1:1–11
Delius M, Enders G, Xuan Z, Liebich HG, Brendel W (1988) Biological effects of shock waves: kidney damage by shock waves in dogs-dose dependence. Ultrasound Med Biol 14:117–122
Denizot F, Lang R (1986) Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J Immunol Methods 89:271–277
Drach GW, Dretler S, Fair W, Finlayson B, Gillenwater J, Griffith D, Lingeman J, Newman D (1986) Report of the United States cooperative study of extracorporeal shock wave lithotripsy. J Urol 135:1127–1133
Kaude JV, Williams CM, Millner MR, Scott KN, Finlayson B (1985) Renal morphology and function immediately after extracorporeal shock-wave lithotripsy. AJR 145:305–313
Lane P, Vichi P, Bain DL, Tritton TR (1987) Temperature dependence studies of Adriamycin uptake and cytotoxicity. Cancer Res 47:4038–4042
Law LW, Dunn TB, Boyle PJ, Miller JH (1949) Observations on the effect of a folic-acid antagonist on transplantable lymphoid leukemias in mice. J Natl Cancer Inst 10:179–192
Levin M, Silber R, Israel M, Goldfeder A, Ketharpal VK, Potmesil M (1981) Protein-associated DNA breaks and DNA-protein cross-links caused by DNA nonbinding derivatives of Adriamycin in L1210 cells. Cancer Res 41:1006–1010
Momparler RL, Karon M, Siegel SE, Avila F (1976) Effect of Adriamycin on DNA, RNA, and protein synthesis in cell-free systems and intact cells. Cancer Res 36:2891–2895
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63
Pinto AL, Lippard SJ (1985) Binding of the antitumor drug cis-diamminedichloroplatinum (II) (cisplatin) to DNA. Biochim Biophys Acta 780:167–180
Richon VM, Schulte N, Eastman A (1987) Multiple mechanisms of resistance to cis-diamminedichloroplatinum (II) in murine leukemia L1210 cells. Cancer Res 47:2056–2061
Rubin JI, Arger PH, Pollack HM, Banner MP, Coleman BG, Mintz MC, VanArsdalen KN (1987) Kidney changes after extracorporeal shock wave lithotripsy: CT evaluation. Radiology 162:21–24
Russo P, Stephenson RA, Mies C, Huryk R, Heston WDW, Melamed MR, Fair WR (1986) High energy shock waves suppress tumor growth in vitro and in vivo. J Urol 135:626–628
Russo P, Mies C, Huryk R, Heston WDW, Fair WR (1987) Histopathologic and ultrastructural correlates of tumor growth suppression by high energy shock waves. J Urol 137:338–341
Sauerbruch T, Delius M, Paumgartner G, Holl J, Wess O, Weber W, Hepp W, Brendel W (1986) Fragmentation of gallstones by extracorporeal shock waves. N Engl J Med 314:818–822
Siegfried JM, Kenndy KA, Sartorelli AC, Tritton TR (1983) The role of membranes in the mechanism of action of the antineoplastic agent Adriamycin. J Biol Chem 258:339–343
Siegfried JM, Burke TG, Tritton TR (1985) Cellular transport of anthracyclines by passive diffusion. Implications for drug resistance. Biochem Pharmacol 34:593–598
Skovsgaard T, Nissen NI (1982) Membrane transport of anthracyclines. Pharmacol Ther 18:293–311
Stephens RJ, Hart CP, Torbit CA, Edmonds PD (1980) Reproductible subcellular alterations in hepatocytes resulting from ultrasound. Ultrasound Med Biol 6:239–249
Tritton TR, Yee G (1982) The anticancer agent Adriamycin can be actively cytotoxic without entering cells. Science 217:248–250
Zwelling LA, Anderson T, Kohn KW (1979) DNA-protein and DNA interstrand cross-linking by cis- and trans-platinum (II) diamminedichloride in L1210 mouse leukemia cells and relation to cytotoxicity. Cancer Res 39:365–369
Author information
Authors and Affiliations
Additional information
Supported by the Kurt Körber Stiftung
Rights and permissions
About this article
Cite this article
Wilmer, A., Gambihler, S., Delius, M. et al. In vitro cytotoxic activity of lithotripter shock waves combined with Adriamycin or with cisplatin on L1210 mouse leukemia cells. J Cancer Res Clin Oncol 115, 229–234 (1989). https://doi.org/10.1007/BF00391694
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00391694