Summary
Organized tissue fragments obtained by dissociation of a pure mucoid carcinoma of the breast were cultured in vitro. The cellular organization of the fragments appcars similar to that observed in vivo, and consist of mucous-filled spherules surrounded by a single layer of tumour cells. Time-lapse video recordings revealed that the cells surrounding these fragments undergo a concerted contraction and relaxation over the time span of several hours. The presence of cytokeratin and oestrogen receptors in the cells suggests an epithelial derivation. Movement of tumour fragments of the type described here could potentially influence the metastatic process of some breast cancers.
References
Charpin C, Martin PM, De Victor B, Lavaut MN, Habid MC, Andrac L, Toga M (1988) Multiple parametric study (Samba 200) of estrogen receptor immunocytochemical assay in four hundred breast carcinomas: analysis of estrogen receptor distribution heterogeneity in tissues and correlations with dextran coated charco assays and morphological data. Cancer Res 48:1578–1586
Clayton F (1986) Pure mucinous carcinomas of the breast: morphologic features and prognostic correlates. Hum Pathol 17:34–38
Gad A, Azzopardi JG (1975) Lobular carcinoma of the breast: a special variant of mucin-secreting carcinoma. J Clin Pathol 28:711–716
Ozello L, Derosa CM, Konrath JG, Yager JL, Miller LS (1986) Detection of estrophillin in frozen sections of breast cancers using an estrogen receptor immunocytochemical assay. Cancer Res 46:4303s-4307s
Rasmussen BB (1985) Human mucinous breast carcinomas and their lymph node metastases. A histological review of 247 cases. Pathol Res Pract 180:377–382
Schmidts HL, Muller H, Sakuma T, Hubner K, Stutte HJ (1985) Enzymhistochemische und immunohistochemische Untersuchungen an der epithelialen Komponente des Mamma-carcinomos. Verh Dtsch Ges Pathol 69:346–349
Silverberg SG, Kay S, Chitale AR, Levitt SH (1971) Colloid carcinoma of the breast. Am J Clin Pathol 55:355–363
Van Netten JP, Thornton IG, Carlyle SJ, Brigden ML, Coy P, Goodchild NL, Gallagher S, George EJ (1987) Multiple microsample analysis of intratumor estrogen receptor distribution in breast cancers by a combined biochemical/immunohistochemical method. Eur J Cancer Clin Oncol 23:1337–1442
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Supported by a grant from the Medical Research Council of Canada
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van Netten, J.P., Racca, R.L., Ashwood-Smith, M.J. et al. Concerted contractions of tumour fragments derived from a pure mucoid carcinoma of the breast in vitro. J Cancer Res Clin Oncol 114, 523–524 (1988). https://doi.org/10.1007/BF00391505
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DOI: https://doi.org/10.1007/BF00391505