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Hydrogen-peroxide-scavenging systems within pea chloroplasts

A quantitative study

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Abstract

The subcellular distribution of ascorbate peroxidase and glutathione reductase (EC 1.6.4.2) in pea leaves was compared with that of organelle markers. Enzyme distribution was found to be similar to that of the chloroplast enzyme NADPH-glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13). Isolated chloroplasts showed a close correlation between intactness and the percentage of enzyme activity recovered. Chloroplasts of 85% intactness were found to contain a high proportion of leaf dehydroascorbate reductase activity (EC 1.8.5.1), 10% of leaf glutathione and 30% of leaf ascorbate. These results are discussed in relation to the potential role of chloroplast antioxidant systems in plant resistance to environmental and other stress conditions.

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Abbreviations

GSH:

reduced glutathione

GSSG:

oxidized glutathione

NADPH-GPD:

glyceraldehyde-3-phosphate dehydrogenase

SOD:

superoxide dismutase

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Authors and Affiliations

  1. School of Biological Sciences, University of Bath, Claverton Down, BA2 7AY, Bath, UK

    D. J. Gillham & A. D. Dodge

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  1. D. J. Gillham
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  2. A. D. Dodge
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Gillham, D.J., Dodge, A.D. Hydrogen-peroxide-scavenging systems within pea chloroplasts. Planta 167, 246–251 (1986). https://doi.org/10.1007/BF00391422

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  • DOI: https://doi.org/10.1007/BF00391422

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