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Immunochemical studies on xanthine dehydrogenase of soybean root nodules

Ontogenic changes in the level of enzyme and immunocytochemical localization

Planta volume 167, pages 190–195 (1986)Cite this article

Abstract

Xanthine dehydrogenase (XDH, EC 1.2.1.37) was purified from root nodules of soybean (Glycine max) and used to prepare a polyclonal rabbit antiserum. Monospecificity of this antiserum was ascertained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the immunoprecipate. During root nodule development of soybean, only one form of XDH was detected on an immunological basis. Titration of XDH by immunoelectrophoresis showed that a remarkable increase in the amount of XDH occurred between two and four weeks after inoculation, in parallel with the increase in enzyme activity. Localization of XDH by immunofluorescence indicated that the enzyme was present exclusively in uninfected cells where it appeared to be associated with discrete organellels

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Abbreviations

IgG:

immunoglobulin G

SDS-PAGE:

sodium dodecyl sulfate — polyacrylamide gel electrophoresis

XDH:

xanthine dehydrogenase

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Author information

Affiliations

  1. Institut de Physiologie Végétale, Centre National de la Recherche Scientifique, F-91190, Gif-sur-Yvette, France

    J. Nguyen & L. Machal

  2. Physiologie Végétale Moléculaire, UA 1128 CNRS, Université Paris-Sud, Bât. 430, F-91405, Orsay Cedex, France

    J. Vidal & P. Gadal

  3. Structure et Métabolisme des Plantes, UA 1128 CNRS, Université Paris-Sud, Bât. 430, F-91405, Orsay Cedex, France

    C. Perrot-Rechenmann

Authors
  1. J. Nguyen
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  2. L. Machal
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  3. J. Vidal
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  4. C. Perrot-Rechenmann
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  5. P. Gadal
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Nguyen, J., Machal, L., Vidal, J. et al. Immunochemical studies on xanthine dehydrogenase of soybean root nodules. Planta 167, 190–195 (1986). https://doi.org/10.1007/BF00391414

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  • DOI: https://doi.org/10.1007/BF00391414

Key words

  • Glycine (xanthine dehydrogenase)
  • Immunocytochemistry
  • Polyclonal antibody
  • Root nodule
  • Xanthine dehydrogenase