Abstract
The ability of vacuoles prepared from V. faba leaves to convert 1-aminocyclopropane-1-carboxylic acid to C2H4 was destroyed when vacuoles were lysed by passage through a hypodermic needle, freezing and thawing, osmotic shock, treatment with ethanol or with a detergent. Ethylene synthesis in the vacuolar fraction was also inhibited by the uncouplers carbonyl cyanide m-chlorophenyl hydrazone and dinitrophenol and by the ionophores valinomycin, nigericin, and A23187. Ethylene formation increased with increasing pH of the incubation medium over the pH range of 5.0–7.5. These observations support the hypothesis that C2H4 biosynthesis in vacuolar preparations is dependent on membrane integrity, possibly because of the requirement for a transmembrane ion gradient.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- ACC:
-
1-aminocyclopropane-1-carboxylic acid
- AVG:
-
aminoethoxyvinylglycine
- CCCP:
-
carbonyl cyanide m-chlorophenyl hydrazone
References
Apelbaum, A., Wang, S.Y., Burgoon, A.C., Baker, J.E., Lieberman, M. (1981) Inhibition of the conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene by structural analogs, inhibitors of electron transfer, uncouplers of oxidative phosphorylation, and free radical scavengers. Plant Physiol. 67, 74–79
Boller, T., Kende, H. (1979) Hydrolytic enzymes in the central vacuole of plant cells. Plant Physiol. 63, 1123–1132
Boudet, A.M., Canut, H., Alibert, G. (1981) Isolation and characterization of vacuoles from Melilotus alba mesophyll. Plant Physiol. 68, 1354–1358
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal. Biochem. 72, 248–254
Guy, M., Kende, H. (1984a) Ethylene formation in Pisum sativum and Vicia faba protoplasts. Planta 160, 276–280
Guy, M., Kende, H. (1984b) Conversion of 1-aminocyclopropane-1-carboxylic acid to ethylene by isolated vacuoles of Pisum sativum L. Planta 160, 281–287
Heytler, P.G. (1979) Uncouplers of oxidative phosphorylation. Methods Enzymol. 55, 462–472
John, P. (1983) Hypothesis: The coupling of ethylene biosynthesis to a transmembrane, electrogenic proton flux. FEBS Lett. 152, 141–143
Kende, H., Hanson, A.D. (1976) Relationship between ethylene evolution and senescence in morning-glory flower tissue. Plant Physiol. 57, 523–527
Lizada, M.C.C., Yang, S.F. (1979) A simple and sensitive assay for 1-aminocyclopropane-1-carboxylic acid. Anal. Biochem. 100, 140–144
McKeon, T.A., Yang, S.F. (1984) A comparison of the conversion of 1-amino-2-ethylcyclopropane-1-carboxylic acid to 1-butene by pea epicotyls and a cell-free system. Planta 160, 84–87
O'Neill, S.D., Bennett, A.B., Spanswick, R.M. (1983) Characterization of a NO3 --sensitive H+-ATPase from corn roots. Plant Physiol. 72, 837–846
Reed, P.W. (1979) Ionophores. Methods Enzymol. 55, 435–454
Tolbert, N.E. (1971) Isolation of leaf peroxisomes. Methods Enzymol. 23, 665–682
Venis, M.A. (1984) Cell-free ethylene forming systems lack stereochemical fidelity. Planta 162, 85–88
Wallsgrove, R.M., Lea, P.J., Miflin, B.J. (1979) The distribution of the enzymes of nitrogen assimilation within the pea leaf cell. Plant Physiol. 63, 232–236
Yang, S.F., Hoffman, N.E. (1984) Ethylene biosynthesis and its regulation in higher plants. Annu. Rev. Plant Physiol. 35, 155–189
Yu, Y.-B., Adams, D.O., Yang, S.F. (1980) Inhibition of ethylene production by 2,4-dinitrophenol and high temperature. Plant Physiol. 66, 286–290
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mayne, R.G., Kende, H. Ethylene biosynthesis in isolated vacuoles of Vicia faba L. — requirement for membrane integrity. Planta 167, 159–165 (1986). https://doi.org/10.1007/BF00391410
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00391410