Abstract
Slices from the hepatopancreas of various oceanic curstaceans incorporated radioactivity into wax esters from 14C glucose and 14C aspartic acid to a lesser extent and from 14C palmitic acid to a much greater extent. Radioactivity was incorporated from 14C palmitic acid into both fatty acid and fatty alcohol moieties of wax esters, the percentage of total radioactivity present in alcohol moieties being greater in deep-living than in shallow-living species. Cell-free preparations from the hepatopancreas but not from muscle, supplemented with ATP and reduced pyridine nucleotides, incorporated radioactivity from 1-14C palmitoyl Coenzyme A into both fatty alcohol and fatty acid moieties of wax esters. Incorporation into fatty alcohol was NADPH- rather than NADH-specific. Preparations from deep-living species had a greater percentage of total radioactivity in the fatty alcohol moieties of wax esters than preparations from shallow-living species. We conclude that the level of wax esters in a given species is correlated with the rate at which the species biosynthesises these lipids de novo; deep-living species have higher rates of wax ester biosynthesis and higher levels of wax esters than shallow-living species. The results support the thesis that wax esters in oceanic crustaceans are derived largely from the animals' internal biosynthetic activities, presumably in response to particular biochemical and/or physiological requirements, rather than from their diets.
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Literature Cited
Baker, A. deC. M.R. Clarke and M.J. Harris: The N.I.O. combination net (RMT 1+8) and further developments of rectangular midwater trawls. J. mar. biol. Ass. U.K. 53, 167–184 (1973)
Benson, A.A., R.F. Lee and J.C. Nevenzel: Wax esters: major marine metabolic energy sources. Biochem. Soc. Symp. 35, 175–187 (1972)
Folch, J., M. Lees and G.H. Sloane Stanley: A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Chem. 226a, 497–509 (1957)
Gatten, R.R. and J.R. Sargent: Wax ester biosynthesis in calanoid copepods in relation to vertical migration. Neth. J. Sea Res. 7, 151–158 (1973)
Hagiwara, S. and T.H. Bullock: Intracellular potentials in pacemaker and intergrative neurones of the lobster cardiac ganglion. J. cell. comp. Physiol. 50, 25–48 (1957)
Kayama, M. and J.C. Nevenzel: Wax ester biosynthesis by midwater marine animals. Mar. Biol. 24, 279–285 (1974)
Lee, R.F. and J. Hirota: Wax esters in tropical zooplankton and nekton and the geographical distribution of wax esters in marine copepods. Limnol. Oceanogr. 18, 227–239 (1973)
—— and A.M. Barnett: Distribution and importance of wax esters in marine copepods and other zooplankton. Deep-Sea Res. 18, 1147–1165 (1971a)
—, J.C. Nevenzel and G.-A. Paffenhöfer: Importance of wax esters and other lipids in the marine food chain: phytoplankton and copepods. Mar. Biol. 9, 99–108 (1971b)
Morris, R.J.: The occurrence of wax esters in crustaceans from the North-east Atlantic Ocean. Mar. Biol. 16, 102–107 (1972)
— Changes in the lipid composition of Acanthephyra purpurea Milne Edwards (Crustacea: Decapoda) during its diurnal migration: a preliminary investigation. J. exp. mar. Biol. Ecol. 13, 55–61 (1973)
— and F. Culkin: Marine lipids: analytical techniques and fatty acid analyses. Oceanogr. mar. Biol. A. Rev. 14, 391–433 (1976)
— and J.R. Sargent: Studies on the lipid metabolism of some oceanic crustaceans. Mar. Biol. 22, 77–83 (1973)
Nevenzel, J.C.: Occurrence, function and biosynthesis of wax esters in marine organisms. Lipids 5, 308–319 (1970)
Sargent, J.R. and R.R. Gatten: The distribution and metabolism of wax esters in marine invertebrates. Biochem. Soc. Trans. 4, 431–433 (1976)
— and R.F. Lee: Biosynthesis of lipids in zooplankton from Sannich Inlet, British Columbia, Canada. Mar. Biol. 31, 15–23 (1975)
—— and J.C. Nevenzel: Marine waxes. In: Chemistry and biochemistry of natural waxes, pp 50–91. Ed. by P.E. Kolattukudy. Amsterdam: Elsevier 1976
— and R. McIntosh: Studies on the mechanism of biosynthesis of wax esters in Euchaeta norvegica. Mar. Biol. 25, 271–277 (1974)
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Communicated by J. Mauchline, Oban
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Sargent, J.R., Morris, R.J. & McIntosh, R. Biosynthesis of wax esters in oceanic crustaceans. Mar. Biol. 46, 315–320 (1978). https://doi.org/10.1007/BF00391402
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DOI: https://doi.org/10.1007/BF00391402