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Partial isotope fractionation during high-performance liquid chromatography of deuterium-labelled internal standards in plant hormone analysis: A cautionary note

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Abstract

Deuterium-labelled indole-3-acetic acid, abscisic acid and phthalimido-1-aminocyclopropane-1-carboxylic acid were found to separate from the unlabelled compounds on reverse-phase high-performance liquid chromatography (HPLC). A similar separation was found for the methyl esters of these compounds on normal-phase HPLC. Such separations may lead to substantial errors when these compounds are used as internal standards for quantitation by gas chromatography-mass spectrometry/selective ion detection, unless the complete chromatographic peaks are collected.

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Abbreviations

ABA:

abscisic acid

ACC:

1-aminocyclopropane-1-carboxylic acid

IAA:

indole-3-acetic acid

GC-MS:

gas chromatography-mass spectrometry

HPLC:

high-performance liquid chromatography

Pht-ACC:

phthalimido-ACC

SIM:

selected ion monitoring

References

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Authors and Affiliations

  1. Department of Botany and Microbiology, University College of Wales, SY23 3DA, Aberystwyth, UK

    B. H. Brown, S. J. Neill & R. Horgan

Authors
  1. B. H. Brown
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  2. S. J. Neill
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  3. R. Horgan
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Brown, B.H., Neill, S.J. & Horgan, R. Partial isotope fractionation during high-performance liquid chromatography of deuterium-labelled internal standards in plant hormone analysis: A cautionary note. Planta 167, 421–423 (1986). https://doi.org/10.1007/BF00391349

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  • DOI: https://doi.org/10.1007/BF00391349

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