Skip to main content
SpringerLink
Log in

Evidence for the involvement of a UDP-glucose-dependent group translocator in sucrose uptake into vacuoles of storage roots of red beet

  • Published:
Planta Aims and scope Submit manuscript

Abstract

Vacuoles isolated from the storage roots of red beet (Beta vulgaris L.) accumulate sucrose via two different mechanisms. One mechanism transports sucrose directly, and its rate is increased by the addition of MgATP. The other mechanism utilizes uridine diphosphate glucose (UDP-glucose) to synthesize and simultaneously transport sucrose phosphate and sucrose into the vacuole. This group translocation mechanism has also been found in sugarcane vacuoles. As in sugarcane, the beet group translocator does not require fructose 6-phosphate, nor is the latter substance transported into the vacuole. The uptake of UDP[14C]glucose in inhibited by high concentrations of osmoticum.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

EDTA:

ethylenediaminetetraacetic acid

Hepes:

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid

UDP:

uridine 5′-diphosphate

References

  • Bennett, A.B., O'Neill, S.D., Spanswick, R.M. (1984) H+-ATPase activity from storage tissue of Beta vulgaris. I. Identification and characterization of an anion-sensitive H+-ATPase. Plant Physiol. 74, 538–544

    Google Scholar 

  • Briskin, D.P., Thornley, W.R., Wyse, R.E. (1985) Membrane transport in isolated vesicles from sugarbeet taproot. II. Evidence for a sucrose/H+ antiport. Plant Physiol. 78, 871–875

    Google Scholar 

  • Brown, S.C., Coombe, B.G. (1984) Proposal for hexose group translocation at the tonoplast of grape pericarp cells. Physiol. Vég. 22, 231–240

    Google Scholar 

  • Doll, S., Rodier, P., Willenbrink, J. (1979) Accumulation of sucrose in vacuoles isolated from red beet tissue. Planta 144, 407–411

    Google Scholar 

  • Jones, M.G.K., Outlaw, W.H.Jr., Lowry, O.H. (1977) Enzymic assay of 10−7 to 10−14 moles of sucrose in plant tissues. Plant Physiol. 60, 379–383

    Google Scholar 

  • Leigh, R.A. (1984) The role of the vacuole in the accumulation and mobilization of sucrose. Plant Growth Regul 2, 339–346

    Google Scholar 

  • Leigh, R.A., ap Rees, T., Fuller, W.A., Banfield, J. (1979) The location of acid invertase activity and sucrose in the vacuoles of storage root of beetroot (Beta vulgaris). Biochem. J. 178, 539–547

    Google Scholar 

  • Leigh, R.A., Branton, D. (1976) Isolation of vacuoles from root storage tissue of Beta vulgaris L. Plant Physiol. 58, 656–662

    Google Scholar 

  • Milford, G.F.J. (1973) The growth and development of the storage root of sugar beet. Ann. Appl. Biol. 75, 427–438

    Google Scholar 

  • Poole, R.J., Briskin, D.P., Kratky, Z., Johnstone, R.M. (1984) Density gradient localization of plasma membrane and tonoplast from storage tissue of growing and dormant red beet. Plant Physiol. 74, 749–756

    Google Scholar 

  • Saftner, R.A., Daie, J., Wyse, R.E. (1983) Sucrose uptake and compartmentation in sugar beet taproot tissue. Plant Physiol. 72, 1–6

    Google Scholar 

  • Saftner, R.A., Wyse, R.E. (1980) Alkali cation/sucrose co-transport in the root sink of sugar beet. Plant Physiol. 66, 884–889

    Google Scholar 

  • Thom, M., Komor, E. (1984a) Role of the ATPase of sugarcane vacuoles in energization of the tonoplast. Eur. J. Biochem. 138, 93–99

    Google Scholar 

  • Thom, M., Komor, E. (1984b) H+-sugar antiport as the mechanism of sugar uptake by sugarcane vacuoles. FEBS Lett. 173, 1–4

    Google Scholar 

  • Thom, M., Komor, E., Maretzki, A. (1982a) Vacuoles from sugarcane cultures. II. Characterization of sugar uptake. Plant Physiol. 69, 1320–1325

    Google Scholar 

  • Thom, M., Maretzki, A. (1985) Group translocation as a mechanism for sucrose transfer into vacuoles from sugarcane cells. Proc. Natl. Acad. Sci. USA 82, 4697–4701

    Google Scholar 

  • Thom, M., Maretzki, A., Komor, E. (1982b) Vacuoles from sugarcane cultures. I. Isolation and partial characterization. Plant Physiol. 69, 1315–1319

    Google Scholar 

  • Walker, R.R., Leigh, R.A. (1981) Characterization of a salt-stimulated ATPase activity associated with vacuoles isolated from storage roots of red beet (Beta vulgaris L.). Planta 153, 140–149

    Google Scholar 

Download references

Author information

Author notes

  1. R. A. Leigh

    Present address: Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., UK

Authors and Affiliations

  1. Experiment Station, Hawaiian Sugar Planters' Association, P.O. Box 1057, 96701, Aiea, HI, USA

    M. Thom, R. A. Leigh & A. Maretzki

Authors
  1. M. Thom
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. A. Leigh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Maretzki
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Thom, M., Leigh, R.A. & Maretzki, A. Evidence for the involvement of a UDP-glucose-dependent group translocator in sucrose uptake into vacuoles of storage roots of red beet. Planta 167, 410–413 (1986). https://doi.org/10.1007/BF00391347

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00391347

Key words

Search

Navigation