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Light-promoted diffusional amino acid efflux from Commelina leaf disks

Indirect control by proton pump activities

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Abstract

The release (=the measured loss) of amino acids was studied in Commelina benghalensis leaf disks. The release is assumed to be the result of influx and efflux, therefore, both movements were investigated.

The uptake of 14C-labeled valine exhibited a biphasic isotherm. The uptake was pH-dependent, especially at low substrate concentrations (pH optimum 4.8). Signals for amino acid/proton co-transport were observed: stimulation of the uptake by fusicoccin (FC), inhibition by diethylstilbestrol (DES) or by high K+ concentrations. In the light, the ATP level of the disks was maintained during the uptake period (2 h), in darkness the ATP content decreased from 87 to 24 nmol g−1 fr. wt. However, light-promoted uptake, which is explained in the proton pump concept by an intensified proton extrusion as the result of high ATP production, was lacking.

The release of amino acids was increased by washing with p-chloromercuriphenyl sulphonic acid (PCMBS), nystatin, 3(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), or KCN. The release (Q10 about 1.5) was independent of the external pH and was linearly related to the intracellular amino acid concentration. Light enhanced the rate of release to the same extent at all intracellular concentrations. The present results suggest that the release is balanced by a, at least partially, proton-driven influx and a diffusional ligh-promoted efflux. A provisional model shows how the diffusional effulx can be indirectly controlled by a counter-flow fueled by the metabolism.

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Abbreviations

PCMBS:

p-chloromercuriphenylsulphonic acid

CCCP:

carbonyl cyanide m-chlorophenyl hydrazone

DES:

diethylstibestrol

DCMU:

3 (3,4-dichlorophenyl)-1, 1-dimethyl urea

TRIS:

2-amino-2-(hydromethyl)propane-1,3 diol

MES:

2-(N1-morpholino) ethane sulphonic acid monohydrate

FC:

fusicoccin

References

  • Buggeln, R.G., Luken, S. (1980) Kinetic characteristics of photoassimilate translocation in Alaria esculenta (Laminariales, Phaeophyceae). Planta 147, 241–245

    Google Scholar 

  • Cataldo, D.A. (1974) Vein loading: the role of the symplast in intercellular transport of carbohydrate between the mesophyll and minor veins of tobacco leaves. Plant Physiol. 53, 912–917

    Google Scholar 

  • Cheung, Y.-N.S., Nobel, P.S. (1973) Amino acid uptake by pea leaf fragments. Specificity, energy sources, and mechanism. Plant Physiol. 52, 633–637

    Google Scholar 

  • Contardi, P.J., Davis, R.F. (1978) Membrane potential in Phaeoceros laevis. Plant Physiol. 61, 164–169

    Google Scholar 

  • Delrot, S., Bonnemain, J.-L. (1978) Etude du mécanisme des produits de la photosynthèse dans les nervures. C.R. Acad. Sci. Paris 287D, 125–130

    Google Scholar 

  • Delrot, S., Bonnemain, J.-L. (1979) Echanges H+−Rb+ et cotransport H+ - glucide dans les tissues foliaires de Vicia faba L. C.R. Acad. Sci. Paris 288D, 71–76

    Google Scholar 

  • Despeghel, J.-P., Delrot, S., Bonnemain, J.-L., Besson, J. (1980) Etude du mécanisme de l'absorption des acides aminés par les tissues foliaires du Vicia faba. C.R. Acad. Sci. Paris 290D, 609–614

    Google Scholar 

  • Doman, D.C., Geiger, D.R. (1979) Effect of exogeneously supplied foliar potassium on phloem loading in Beta vulgaris. Plant Physiol. 64, 528–533

    Google Scholar 

  • Fischer, E., Lüttge, U. (1980) Membrane potential changes related to active transport of glycine in Lemna gibba Gl. Plant Physiol. 65, 1004–1008

    Google Scholar 

  • Geiger, D.R. (1976) Phloem loading in source leaves. In: Transport and transfer processes in plants, pp. 167–183. Wardlaw, I.F., Passioura, J.B. eds. Academic Press, New York San Francisco London

    Google Scholar 

  • Geiger, D.R., Giaquinta, R.T., Sovonick, S.A., Fellows, R.J. (1973) Solute distribution in sugar beet leaves in relation to phloem loading and translocation. Plant Physiol. 52, 585–589

    Google Scholar 

  • Giaquinta, R.T. (1979) Phloem loading of sucrose. Involvement of membrane ATP-ase and proton transport. Plant Physiol. 63, 744–748

    Google Scholar 

  • Giersch, C., Heber, U., Krause, G.H. (1980) ATP-transfer from chloroplasts to the cytosol of leaf cells during photosynthesis and its effect on leaf metabolism. In: Plant membrane transport: Current conceptual issues, pp. 65–82, Spanswick, R.M., Lucas, W.J., Daintly, J. eds. Elsevier/North Holland Biomedical Press, Amsterdam New York Oxford

    Google Scholar 

  • Gordon, A.J., Ryle, G.J.A., Powell, C.E., Mitchell, D. (1980) Export, mobilization and respiration of assimilates in uniculm barley during light and darkness. J. Exp. Bot. 31, 461–473

    Google Scholar 

  • Gunning, B.E.S. (1976) The role of plasmodesmata in short distance transport to and from the phloem. In: Intracellular communication in plants: Studies on plasmodesmata, pp. 203–227, Gunning, B.E.S., Robards, a.W., eds. Springer Verlag, Berlin Heidelberg New York

    Google Scholar 

  • Guy, M., Reinhold, L., Rahat, M. (1980) Energization of the sugar transport mechanism in the plasmalemma of isolated mesophyll protoplasts. Plant Physiol. 65, 550–553

    Google Scholar 

  • Hartt, C.E. (1965) Light and translocation of 14C in detached blades of sugar cane. Plant Physiol. 40, 718–724

    Google Scholar 

  • Heyser, W. (1980) Phloem loading in the maize leaf. Ber. Dtsch. Bot. Ges. 93, 221–228

    Google Scholar 

  • Huber, S.C., Moreland, D.E. (1980) Translocation. Efflux of sugars across the plasmalemma of mesophyll protoplasts. Plant Physiol. 65, 560–562

    Google Scholar 

  • Jeschke, W.D. (1970) Lichtabhängige Veränderungen des Membranpotentials bei Blattzellen von Elodea densa. Z. Pflanzenphysiol. 62, 158–172

    Google Scholar 

  • Kaiser, W.M., Paul, J.S., Bassham, J.A. 1979) Release of photosynthates from mesophyll cells in vitro and in vivo. Z. Pflanzenphysiol. 94, 377–385

    Google Scholar 

  • Keifer, D.W., Spanswick, R.M. (1979) Correlation of adenosine triphosphate levels in Chara corallina with the activity of the electrogenic pump. Plant Physiol. 64, 165–168

    Google Scholar 

  • Komor, E., Rotter, M., Waldhauser, J., Martin, E., Cho, B.H. (1980) Sucrose proton symport for phloem loading in the Ricinus seedling. Ber. Dtsch. Bot. Ges. 93, 211–219

    Google Scholar 

  • Komor, E., Tanner, W. (1974) The hexose-proton co-transport system of Chlorella. J. Gen. Physiol. 64, 568–581

    Google Scholar 

  • Komor, E., Weber, H., Tanner, W. (1978) Essential sulfhydryl group in the transport-catalyzing protein of the hexose-proton cotransport system of Chlorella. Plant Physiol. 61, 785–786

    Google Scholar 

  • Kursanov, A.L., Brovchenko, M.I. (1970) Sugars in the free space of leaf plate: their origin and possible involvement in transport. Can. J. Bot. 48, 1243–1250

    Google Scholar 

  • Lien, R., Rognes, S.E. (1977) Uptake of amino acids by barley leaf slices: Kinetics, specificity, and energetics. Physiol. Plant. 41, 175–183

    Google Scholar 

  • Lüttge, U., Pallaghy, C.K. (1969) Light-triggered transient changes of membrane potentials in green cells in relation to photosynthetic electron transport. Z. Pflanzenphysiol. 61, 58–67

    Google Scholar 

  • Novacky, A., Karr, A.L. (1977) Pathological alterations in cell membrane electrical properties. In: Regulation of cell membrane activities in plants, pp. 137–144, Marrè, E., Ciferri, O., eds. Elsevier/North Holland Biomedical Press, Amsterdam

    Google Scholar 

  • Novacky, A., Ullrich-Eberius, C.I., Lüttge, U. (1978) Membrane potential changes during transport of hexoses in Lemna gibba Gl. Planta 138, 263–270

    Google Scholar 

  • Pate, J.S., Gunning, B.E.S. (1972) Transfer cells. Annu. Rev. Plant Physiol. 23, 173–196

    Google Scholar 

  • Penth, B., Weigl, J. (1971) Anionen-Influx, ATP-Spiegel und CO2-Fixierung in Limnophila gratioloides und Chara foetida. Planta 96, 212–223

    Google Scholar 

  • Petzold, U., Jacob, F. (1975) Versuche zur Aufnahme xenobiontischer Stoffe durch Blätter von Egeria densa Planchon. I Die Aufnahme von alpha-Aminoisobuttersäure und der Lichteinfluß auf diesen Vorgang. Biol. Zentralbl. 94, 167–185

    Google Scholar 

  • Prins, H.B.A., Harper, J.R., Higinbotham, N. (1980) Membrane potentials of Vallisneria leaf cells and their relation to photosynthesis. Plant Physiol. 65, 1–5

    Google Scholar 

  • Servaites, J.C., Scharader, L.E., Jung, D.M. (1979) Energy-dependent loading of amino acid and sucrose into the phloem of soybean. Plant Physiol. 64, 546–550

    Google Scholar 

  • Shtarkshall, R.A., Reinhold, L., Harel, H. (1970) Transport of amino acids in barley leaf tissue. I Evidence for a specific uptake mechanism and the influence of “ageing” on accumulatory capacity. J. Exp. Bot. 21, 915–925

    Google Scholar 

  • Sinclair, J. (1968) The influence of light on the ion fluxes and electrical potential of the leaf cells of the moss Hookeria lucens. J. Exp. Bot 19, 254–263

    Google Scholar 

  • Van Bel, A.J.E. (1976) Different mass transfer rates of labeled sugars and tritiated water in the xylem vessels and their dependency on metabolism. Plant Physiol. 57, 911–914

    Google Scholar 

  • Van Bel, A.J.E., Van der Schoot, C. (1980) Light-stimulated biphasic amino acid uptake by xylem parenchyma cells. Plant Sci. Lett. 19, 101–107

    Google Scholar 

  • Van Bel, A.J.E., Van Erven, A.J. (1979) A model for proton and potassium co-transport during the uptake of glutamine and sucrose by tomato internode disks. Planta 145, 77–82

    Google Scholar 

  • Van Die, J. (1958) On the occurrence of alpha-keto acids and organic nitrogen compounds in xylem exudates of cucumber and tomato plants. Proc. Kon. Ned. Ak. Wet. C 61, 572–578

    Google Scholar 

  • Weigl, J. (1967) Beweis für die Beteiligung von beweglichen Transportstrukturen (Trägern) beim Ionentransport durch pflanzliche Membranen und die Kinetik des Anionentransports bei Elodea densa im Licht und Dunkeln. Planta 75, 327–342

    Google Scholar 

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  1. Botanisch Laboratorium van de Rijksuniversiteit, Lange Nieuwstraat 106, 3512 PN, Utrecht, The Netherlands

    Aart J. E. van Bel & Ankie Ammerlaan

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  1. Aart J. E. van Bel
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  2. Ankie Ammerlaan
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van Bel, A.J.E., Ammerlaan, A. Light-promoted diffusional amino acid efflux from Commelina leaf disks. Planta 152, 115–123 (1981). https://doi.org/10.1007/BF00391182

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