Abstract
Fructose-1,6-bisphosphatase was isolated from the cyanobacterium Synechococcus 6301 by acid precipitation, ammonium-sulfate fractionation, and Sephadex gel chromatography. The purified enzyme needed thiols and MgCl2 for activity. The following Km-values were obtained: a) for fructose-1,6-bisphosphate: 1.7 mM; b) for MgCl2: 12.5 mM; c) for dithiocrythritol: 0,56 mM; d) for glutathione: 14 mM; e) for mercaptoethanol: 22 mM; f) for cysteine: 50 mM. Thioredoxin B isolated from this organism will activate this fructose-1,6-bisphosphatase. The Km of thioredoxin B for this fructose-1,6-bisphosphatase was determined to be 1.7 μM, endicotiy that thioredoxin might activate the fructose-1,6-bisphosphatase in Synechococcus in vivo.
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Anderson, L.E., Chin, H.M., Gupta, V.K. (1979) Modulation of chloroplast fructose-1-6-bisphosphatase activity by light. Plant Physiol. 64, 191–194
Buchanan, B.B., Schürmann, P., Kalberer, P.P. (1971) Ferredoxinactivated fructose diphosphatase of spinach chloroplasts. J. Biol. Chem. 246, 5952–5959
Buchanan, B.B., Wolosiuk, R.A., Schürmann, P. (1979) Thioredoxin and enzyme regulation. Trends in Biochem. Sciences 4, 93–96
Crawford, N.A., Yee, B.C., Nishizawa, A.N., Buchanan, B.B. (1979) Occurence of cytoplasmatic f- and m-type thioredoxins in leaves. FEBS Lett. 104, 141–145
Duggan, J.X., Anderson, L.E. (1975) Light-regulation of enzyme activity in Anacystis nidulans (Richt.) Planta 122, 293–297
Gleason, F.K. (1978) Distribution of ribonucleotide reductase in the genus Synechococcus. FEMS Microbiol. Lett. 3, 241–243
Jaquot, J-P., Vidal, J., Gadal, P., Schürmann, P. (1978) Evidence for the existence of several enzyme-specific thioredoxins in plants. FEBS Lett. 96, 243–246
Lowry, O.H., Rosebrough, N.J., Farr, A.J., Randall, R.J. (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275
Peschek, G. (1979) The role of the calvin cycle for anoxygenic CO2 photoassimilation in Anacystis nidulans. FEBS Lett. 106, 34–38
Schmidt, A. (1975) Distribution of APS-sulfotransferase activity among higher plants. Plant Sci. Lett. 5, 407–415
Schmidt, A. (1979) A thioredoxin-activated fructose-bisphosphatase in the cyanobacterium Synechococcus 6301. Plant Physiol. 63, S-11
Schmidt, A. (1980) Isolation of two thioredoxins from the cyanobacterium Synechococcus 6301. Arch. Microbiol. 127, 259–265
Schmidt, A., Christen, U. (1978) A factor-dependent sulfortransferase specific for 3′-phosphoadenosine-5′-phosphosulfate (PAPS) in the cyanobacterium Synechococcus 6301. Planta 140, 239–244
Schmidt, A., Christen, U. (1979) Distribution of thioredoxins in cyanobacteria. Z. Naturforsch. 34c, 1272–1274
Stanier, R.Y., Kunisawa, R., Mandel, M., Cohen-Bazire, G. (1971) Purification and properties of unicellular blue-green algae (Order Chroococcales). Bacteriol. Rev. 35, 171–205
Wagner, W. (1978) Thioredoxine aus der Grünalge Scenedesmus obliquus und die multiplen Funktionen von Thioredoxinen in Enzymsystemen. Thesis, Marburg, FRG
Wagner, W., Follmann, H., Schmidt, A. (1987) Multiple functions of thioredoxins. Z. Naturforsch. 33c, 517–520
Wolosiuk, R.A., Buchanan, B.B. (1977) Thioredoxine and glutathione regulate photosynthesis in chloroplasts. Nature (London) 266, 565–567
Wolosiuk, R.A., Crawford, N.A., Yee, B.C., Buchanan, B.B. (1979) Isolation of three thioredoxins from spinach leaves. J. Biol. Chem. 254, 1627–1632
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Schmidt, A. A thioredoxin-activated fructose-1,6-bisphosphatase from the cyanobacterium Synechococcus 6301. Planta 152, 101–104 (1981). https://doi.org/10.1007/BF00391180
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DOI: https://doi.org/10.1007/BF00391180