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Non-photochemical quenching of chlorophyll a fluorescence in isolated chloroplasts under conditions of stressed photosynthesis

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Abstract

Non-photochemical quenching of chlorophyll a fluorescence after short-time light, heat and osmotic stress was investigated with intact chloroplasts from Spinacia oleracea L. The proportions of non-photochemical fluorescence quenching (q N ) which are related (q E ) and unrelated (q I ) to the transthylakoid proton gradient (ΔpH) were determined. Light stress resulted in an increasing contribution of q Ito total q N.The linear dependence of q. Eand ΔpH, as seen in controls, was maintained. The mechanisms underlying this type of quenching are obviously unaffected by photoin-hibition. In constrast, q Ewas severely affected by heat and osmotic stress. In low light, the response of q Eto changes in ΔpH was enhanced, whereas it was reduced in high light. The data are discussed with reference to the hypothesis that q Eis related to thermal dissipation of excitation energy from photosystem II. It is shown that q Eis not only controlled by ΔpH, but also by external factors.

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Abbreviations

9-AA:

9-aminoacridine

F o :

basic chlorophyll fluorescence

F o :

variable chlorophyll fluorescence

L 2 :

saturating light pulse

PS:

photosystem

q E :

ΔpH-dependent, non-photochemical quenching of fluorescence

q I :

ΔpH-independent, non-photochemical quenching

q N :

entire non-photochemical quenching

q Q :

photochemical quenching

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  1. Botanisches Institut der Universität, Universitätsstrasse 1, D-4000, Düsseldorf, Federal Republic of Germany

    Henrik Laasch

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  1. Henrik Laasch
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Laasch, H. Non-photochemical quenching of chlorophyll a fluorescence in isolated chloroplasts under conditions of stressed photosynthesis. Planta 171, 220–226 (1987). https://doi.org/10.1007/BF00391097

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  • DOI: https://doi.org/10.1007/BF00391097

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